Cystic fibrosis (CF) is caused by Cystic FibrosisTransmembrane regulator (CFTR)mutations . The CF clinical variability is associated with CFTR mutations, environmental factors and modifier gene, mainly related to pulmonary disease. Itshould be noted, in this case, the IL-8 gene (interleukin 8), which thepolymorphismhave been related to the CF severity, considering its importanceinimmune response, with a possible effect on the clinical variability of thedisease.This study aims to analyze the distribution of IL-8 polymorphismsin CFpatients, and to evaluate its association with clinical variability of the disease, whereas CFTR mutations.In the study, 190 CF atients will be enrolled, diagnosed bytwo sweat tests with chloride greater than 60 mEq/L and clinical compatibility. TheIL-8 polymorphisms(rs4073, rs2227307 and rs2227306) will be analyzed byPCR/RFLP(polymerase chain reaction/restriction fragment length polymorphism). The patients will also underwent clinical and laboratory study to assessment of clinical severity scores, body mass index, age at diagnosis, 1st clinical symptoms(pulmonary, digestive and both)period to the first colonization by Pseudomonasaeruginosa, microorganisms[P. aeruginosa (mucoid and non-mucoid), Achromobacterxylosoxidans, Burkolderia cepacia and Staphylococcus aureus], spirometry, transcutaneous oxygen saturation and comorbidities (nasalpolyposis, osteoporosis, meconium ileus, diabetes mellitus, pancreatic insufficiency andliver disease). The data will be analyzed considering the level of significance=0.05.
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