| Full text | |
| Author(s): |
Furlan, Larissa Lazzarini
[1]
;
Ribeiro, Jose Dirceu
[2]
;
Bertuzzo, Carmen Silvia
[3]
;
Salomao Junior, Joao Batista
[4, 5]
;
Silva Souza, Doroteia Rossi
[6]
;
Lima Marson, Fernando Augusto
[2, 3]
Total Authors: 6
|
| Affiliation: | [1] Fac Med Sao Jose do Rio Preto, Sao Jose Do Rio Preto, SP - Brazil
[2] Univ Estadual Campinas UNICAMP, Fac Ciencias Med, Dept Pediat, Campinas, SP - Brazil
[3] Univ Estadual Campinas Unicamp, Fac Ciencias Med, Dept Genet Med, Campinas, SP - Brazil
[4] Hosp Univ, Fac Med Sao Jose Do Rio Preto, Dept Pediat, Sao Jose Do Rio Preto, SP - Brazil
[5] Hosp Univ, Fac Med Sao Jose Do Rio Preto, Dept Pneumol Pediat, Sao Jose Do Rio Preto, SP - Brazil
[6] Ctr Pesquisa Bioquim & Biol Mol, Fac Med Sao Jose Do Rio Preto, Dept Biol Mol, Sao Jose Do Rio Preto, SP - Brazil
Total Affiliations: 6
|
| Document type: | Journal article |
| Source: | Jornal de Pediatria; v. 93, n. 6, p. 639-648, NOV-DEC 2017. |
| Web of Science Citations: | 5 |
| Abstract | |
Objective: Interleukin 8 protein promotes inflammatory responses, even in airways. The presence of interleukin 8 gene variants causes altered inflammatory responses and possibly varied responses to inhaled bronchodilators. Thus, this study analyzed the interleukin 8 variants (rs4073, rs2227306, and rs2227307) and their association with the response to inhaled bronchodilators in cystic fibrosis patients. Methods: Analysis of interleukin 8 gene variants was performed by restriction fragment length polymorphism of polymerase chain reaction. The association between spirometry markers and the response to inhaled bronchodilators was evaluated by Mann-Whitney and Kruskal-Wallis tests. The analysis included all cystic fibrosis patients, and subsequently patients with two mutations in the cystic fibrosis transmembrane conductance regulator gene belonging to classes I to III. Results: This study included 186 cystic fibrosis patients. There was no association of the rs2227307 variant with the response to inhaled bronchodilators. The rs2227306 variant was associated with FEF50% in the dominant group and in the group with two identified mutations in the cystic fibrosis transmembrane conductance regulator gene. The rs4073 variant was associated with spirometry markers in four genetic models: co-dominant (FEF25-75% and FEF75%), dominant (FEV1, FEF50%, FEF75%, and FEF25-75%), recessive (FEF75% and FEF25-75%), and over-dominant (FEV1/FVC). Conclusions: This study highlighted the importance of the rs4073 variant of the interleukin 8 gene, regarding response to inhaled bronchodilators, and of the assessment of mutations in the cystic fibrosis transmembrane conductance regulator gene. (C) 2017 Sociedade Brasileira de Pediatria. Published by Elsevier Editora Ltda. (AU) | |
| FAPESP's process: | 11/18845-1 - Association between polymorphisms in modifier genes in children and adolescent with allergic and non-allergic mild, moderate and severe asthma |
| Grantee: | Jose Dirceu Ribeiro |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 15/12183-8 - Identification of prevalent mutations and clinical and functional characterization of children and adults with primary ciliary dyskinesia |
| Grantee: | Jose Dirceu Ribeiro |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 11/12939-4 - Association between polymorphisms in modifier genes in children and adolescent with allergic and non-allergic: mild, moderate and severe asthma |
| Grantee: | Fernando Augusto de Lima Marson |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 13/19052-0 - IL-8 gene as a modifier of cystic fibrosis |
| Grantee: | Larissa Lazzarini Furlan |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |
| FAPESP's process: | 15/12858-5 - Identification of prevalent mutations and clinical and functional characterization of children and adults with primary ciliary dyskinesia |
| Grantee: | Fernando Augusto de Lima Marson |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |