Association between polymorphisms in modifier genes in children and adolescent with allergic and non-allergic mild, moderate and severe asthma

• Grant number: 11/18845-1
• Support Opportunities: Regular Research Grants
• Duration: July 01, 2012 - December 31, 2014
• Field of knowledge: Health Sciences - Medicine - Maternal and Child Health
• Principal Investigator: Jose Dirceu Ribeiro
• Grantee: Jose Dirceu Ribeiro
• Host Institution: Faculdade de Ciências Médicas (FCM). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

Asthma is a chronic inflammatory disease that has a high prevalence with different degrees of severity. Although is not known completely, the genetic and environmental interactions in this disease, but it is known that several genes and environmental factors act on the severity and etiology of asthma. In our study, we selected polymorphisms in modifier genes, previously reported as possible modifeir genes in metabolic pathways that influence in the asthma: ACE and CD14 (pro-inflammatory properties), ALOX5AP LTA4H (active in the biological cycle that leads to the production of LTB4), GCLC and GST [M1, P1 and T1] (active in the glutathione cycle - oxidative stress-related), IL4 [cytokine that induces differentiation of undifferentiated Th cells (Th0) to Th2 cells], IL4R (receptor that binds IL-4 and IL-13 to regulate the production of IgE antibodies), IL13 (acting in allergic inflammation), IL1R1 (expression by pro-inflammatory stimuli and involved in the function of Th cells), NOS-1 (associated with eosinophilic inflammation), STAT6 (activator transcription and serves on the biological response mediated by IL-4), TGF²1 (limited inflammatory reactions and acts in tissue repair and remodeling), TLR2 and TLR4 (recognize foreign substances and transmit signals to the activation of the immune system). Objective: To characterize patients with atopic and nonatopic asthma of the Pediatric Clinic of HC / UNICAMP second questionnaire of clinical severity, determine the frequency of different polymorphisms and associate both with the aim of understanding the presentation of the disease. Method: Cross-sectional study. Will be carried out DNA extraction kit for DNA extraction Flexigene Quiagen Kit ®, analysis of polymorphisms in the ACE gene, GSTM1 and T1 by conventional PCR, genotyping for the NOS-1 gene, and analysis of polymorphisms of SNPs by Open Array technique [OpenArray ® TaqMan ® Genotyping (Applied Biosystems by Life Technologies ®)] of 500 patients with asthma and 500 controls. The classification of severity will be held on the basis DBMA (2006) for all patients. Testing will be performed with logistic and linear regression for each polymorphism in relation to data obtained from patients. The analysis will be performed by the software "Statistical Package for the Social Sciences" (SPSS) v.17.0, with adjustments for multiple tests by Bonferroni test. (AU)

Scientific publications

(34)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PALOMA L.F. PARAZZI; FERNANDO A.L. MARSON; MARIA A.G.O. RIBEIRO; CAMILA I.S. SCHIVINSKI; JOSÉ D. RIBEIRO. Avaliação da dinâmica respiratória por capnografia volumétrica durante o protocolo de exercício submáximo por seis minutos em esteira em pacientes com fibrose cística. Jornal de Pediatria, v. 95, n. 1, p. 76-86, 2019-02-00. (15/12858-5, 15/12183-8, 11/18845-1, 11/12939-4)

SOUZA GOMEZ, CARLA CRISTINA; FRANCISCO PARAZZI, PALOMA LOPES; CLINCKSPOOR, KARL JAN; MAUCH, RENAN MARRICHI; TEIXEIRA PESSINE, FRANCISCO BENEDITO; LEVY, CARLOS EMILIO; PEIXOTO, ANDRESSA OLIVEIRA; GONCALVES OLIVEIRA RIBEIRO, MARIA ANGELA; RIBEIRO, ANTONIO FERNANDO; CONRAD, DOUGLAS; et al. Safety, Tolerability, and Effects of Sodium Bicarbonate Inhalation in Cystic Fibrosis. CLINICAL DRUG INVESTIGATION, NOV 2019. (11/12939-4, 15/12858-5, 15/12183-8, 11/18845-1)

ZIMIANI DE PAIVA, ANA CAROLINA; DE LIMA MARSON, FERNANDO AUGUSTO; RIBEIRO, JOSE DIRCEU; BERTUZZO, CARMEN SILVIA. Asthma: Gln27Glu and Arg16Gly polymorphisms of the beta2-adrenergic receptor gene as risk factors. ALLERGY ASTHMA AND CLINICAL IMMUNOLOGY, v. 10, p. 9-pg., 2014-02-05. (11/18845-1)

MARIANA S. FERREIRA; ROBERTO T. MENDES; FERNANDO A.L. MARSON; MARIANA P. ZAMBON; MARIA A.R.G.M. ANTONIO; ILMA A. PASCHOAL; ADYLÉIA A.D.C. TORO; SILVANA D. SEVERINO; MARIA A.G.O. RIBEIRO; JOSÉ D. RIBEIRO. Espirometria e capnografia volumétrica na avaliação da função pulmonar de indivíduos obesos e eutróficos sem asma. Jornal de Pediatria, v. 93, n. 4, p. 398-405, 2017-08-00. (15/12183-8, 11/18845-1, 11/12939-4, 15/12858-5)

MARSON, FERNANDO A. L.; BERTUZZO, CARMEN S.; RIBEIRO, JOSE D.. Personalized or Precision Medicine? The Example of Cystic Fibrosis. FRONTIERS IN PHARMACOLOGY, v. 8, JUN 20 2017. (11/12939-4, 15/12183-8, 11/18845-1, 15/12858-5)

PEIXOTO, ANDRESSA O.; AL MARSON, FERNANDO; DERTKIGIL, SERGIO SJ; DERTKIGIL, RAQUEL P.; SOUZA, TIAGO H.; FRAGA, ANDREA; RIBEIRO, ANTONIO F.; TORO, ADYLEIA ADC; RIBEIRO, JOSE D.. The Use of Ultrasound as a Tool to Evaluate Pulmonary Disease in Cystic Fibrosis. Respiratory Care, v. 65, n. 3, p. 293-303, MAR 1 2020. (11/12939-4, 15/12183-8, 11/18845-1, 15/12858-5)

SOUZA GOMEZ, CARLA CRISTINA; LIMA MARSON, FERNANDO AUGUSTO; SERVIDONI, MARIA FATIMA; RIBEIRO, ANTONIO FERNANDO; GONCALVES OLIVEIRA RIBEIRO, MARIA ANGELA; LOPES GAMA, VERUSKA ACIOLI; COSTA, EDUARDO TAVARES; RIBEIRO, JOSE DIRCEU; VIEIRA JUNIOR, FRANCISCO UBALDO. Evaluation of continuous constant current and continuous pulsed current in sweat induction for cystic fibrosis diagnosis. BMC PULMONARY MEDICINE, v. 18, SEP 14 2018. (11/12939-4, 15/12858-5, 15/12183-8, 11/18845-1)

COUTINHO, C. A. A. C.; MARSON, F. A. L.; RIBEIRO, J. D.; BERTUZZO, C. S.. A negative screening of rare genetic variants in the ADIPOQ and STATH genes in cystic fibrosis. PULMONOLOGY, v. 26, n. 3, p. 138-144, MAY-JUN 2020. (11/12939-4, 15/12183-8, 11/18845-1, 15/12858-5)

ALMEIDA-JUNIOR, ARMANDO; LIMA MARSON, FERNANDO AUGUSTO; BRESCIANI ALMEIDA, CELIZE CRUZ; GONCALVES OLIVEIRA RIBEIRO, MARIA ANGELA; PASCHOAL, ILMA APARECIDA; MOREIRA, MARCOS MELLO; RIBEIRO, JOSE DIRCEU. Volumetric capnography versus spirometry for the evaluation of pulmonary function in cystic fibrosis and allergic asthma. Jornal de Pediatria, v. 96, n. 2, p. 10-pg., 2020-03-01. (15/12858-5, 11/18845-1, 15/12183-8, 11/12939-4)

PARAZZI, PALOMA L. F.; MARSON, FERNANDO A. L.; RIBEIRO, MARIA A. G. O.; SCHIVINSKI, CAMILA I. S.; RIBEIRO, JOSE D.. Evaluation of respiratory dynamics by volumetric capnography during submaximal exercise protocol o six minutes on treadmill in cystic fibrosis patients. Jornal de Pediatria, v. 95, n. 1, p. 76-86, JAN-FEB 2019. (11/12939-4, 15/12858-5, 15/12183-8, 11/18845-1)

PARAZZI, P. L. F.; MARSON, F. A. L.; RIBEIRO, M. A. G. O.; SCHIVINSKI, C. I. S.; RIBEIRO, J. D.. Correlation between parameters of volumetric capnography and spirometry during a submaximal exercise protocol on a treadmill in patients with cystic fibrosis and healthy controls. PULMONOLOGY, v. 25, n. 1, p. 21-31, JAN-FEB 2019. (11/12939-4, 15/12183-8, 11/18845-1, 15/12858-5)

FARIA, ALETHEA GUIMARAES; LIMA MARSON, FERNANDO AUGUSTO; SOUZA GOMEZ, CARLA CRISTINA; SERVIDONI, MARIA DE FATIMA; RIBEIRO, ANTONIO FERNANDO; RIBEIRO, JOSE DIRCEU. Thirty Years of Sweat Chloride Testing at One Referral Center. FRONTIERS IN PEDIATRICS, v. 5, OCT 26 2017. (11/12939-4, 15/12183-8, 11/18845-1, 15/12858-5)

FURGERI, DANIELA TENORIO; LIMA MARSON, FERNANDO AUGUSTO; ARAUJO CORREIA, CYNTIA ARIVABENI; RIBEIRO, JOSE DIRCEU; BERTUZZO, CARMEN SILVIA. Cystic fibrosis transmembrane regulator haplotypes in households of patients with cystic fibrosis. Gene, v. 641, p. 137-143, JAN 30 2018. (11/12939-4, 15/12858-5, 15/12183-8, 11/18845-1)

FURLAN, LARISSA LAZZARINI; RIBEIRO, JOSE DIRCEU; BERTUZZO, CARMEN SILVIA; SALOMAO JUNIOR, JOAO BATISTA; SILVA SOUZA, DOROTEIA ROSSI; LIMA MARSON, FERNANDO AUGUSTO. Variants in the interleukin 8 gene and the response to inhaled bronchodilators in cystic fibrosis. Jornal de Pediatria, v. 93, n. 6, p. 639-648, NOV-DEC 2017. (11/18845-1, 15/12183-8, 11/12939-4, 13/19052-0, 15/12858-5)

PEREIRA, STEPHANIE VILLA-NOVA; RIBEIRO, JOSE DIRCEU; BERTUZZO, CARMEN SILVIA; LIMA MARSON, FERNANDO AUGUSTO. Association of clinical severity of cystic fibrosis with variants in the SLC gene family (SLC6A14, SLC26A9, SLC11A1 and SLC9A3). Gene, v. 629, p. 117-126, SEP 20 2017. (11/12939-4, 15/12183-8, 11/18845-1, 15/12858-5)

LIMA MARSON, FERNANDO AUGUSTO; BERTUZZO, CARMEN SILVIA; DE ARAUJO, TANIA KAWASAKI; RUSSO HORTENCIO, TAIS DAIENE; RIBEIRO, ANTONIO FERNANDO; RIBEIRO, JOSE DIRCEU. Pancreatic Insufficiency in Cystic Fibrosis: Influence of Inflammatory Response Genes. PANCREAS, v. 47, n. 1, p. 99-109, JAN 2018. (11/12939-4, 15/12183-8, 11/18845-1, 15/12858-5)

CAMARGO BARROS ROCHA, D. A.; MARSON, F. A. L.; ALMEIDA, C. C. B.; ALMEIDA JUNIOR, A. A.; RIBEIRO, J. D.. Association between oxygenation and ventilation indices with the time on invasive mechanical ventilation in infants. PULMONOLOGY, v. 24, n. 4, p. 241-249, JUL-AUG 2018. (11/12939-4, 15/12183-8, 11/18845-1, 15/12858-5)

ALVAREZ, ALFONSO EDUARDO; LIMA MARSON, FERNANDO AUGUSTO; BERTUZZO, CARMEN SILVIA; SANTIAGO BASTOS, JULIANA CRISTINA; ELIAS BARACAT, EMILIO CARLOS; BRANDAO, MARCELO BARCIELA; TRESOLDI, ANTONIA TERESINHA; DAS NEVES ROMANELI, MARIANA TRESOLDI; BRESCIANI ALMEIDA, CELIZE CRUZ; DE OLIVEIRA, THEREZINHA; et al. Association between single nucleotide polymorphisms in TLR4, TLR2, TLR9, VDR, NOS2 and CCL5 genes with acute viral bronchiolitis. Gene, v. 645, p. 7-17, MAR 1 2018. (12/05458-2, 11/18845-1, 15/12858-5)

LARISSA LAZZARINI FURLAN; JOSÉ DIRCEU RIBEIRO; CARMEN SÍLVIA BERTUZZO; JOÃO BATISTA SALOMÃO JUNIOR; DOROTÉIA ROSSI SILVA SOUZA; FERNANDO AUGUSTO LIMA MARSON. Variantes no gene da interleucina 8 e a resposta a broncodilatadores inalatórios na fibrose cística,. Jornal de Pediatria, v. 93, n. 6, p. 639-648, 2017-12-00. (13/19052-0, 11/18845-1, 15/12183-8, 15/12858-5, 11/12939-4)

ARMANDO ALMEIDA-JUNIOR; FERNANDO AUGUSTO LIMA MARSON; CELIZE CRUZ BRESCIANI ALMEIDA; MARIA ÂNGELA GONÇALVES OLIVEIRA RIBEIRO; ILMA APARECIDA PASCHOAL; MARCOS MELLO MOREIRA; JOSÉ DIRCEU RIBEIRO. Capnografia volumétrica versus espirometria para avaliação da função pulmonar na fibrose cística e na asma alérgica,. Jornal de Pediatria, v. 96, n. 2, p. 255-264, 2020-05-11. (11/18845-1, 15/12858-5, 11/12939-4, 15/12183-8)

ZIMIANI DE PAIVA, ANA CAROLINA; DE LIMA MARSON, FERNANDO AUGUSTO; RIBEIRO, JOSE DIRCEU; BERTUZZO, CARMEN SILVIA. Asthma: Gln27Glu and Arg16Gly polymorphisms of the beta2-adrenergic receptor gene as risk factors. ALLERGY ASTHMA AND CLINICAL IMMUNOLOGY, v. 10, FEB 5 2014. (11/18845-1)

UEJIMA SATTO SAKAI, RAQUEL HARUMI; LIMA MARSON, FERNANDO AUGUSTO; INOUE SAKUMA, EMERSON TARO; RIBEIRO, JOSE DIRCEU; SAKANO, EULALIA. Correlação entre rinometria acústica, rinomanometria computorizada e tomografia computadorizada de feixe cônico em respiradores bucais com atresia maxilar. Brazilian Journal of Otorhinolaryngology, v. 84, n. 1, p. 40-50, JAN-FEB 2018. (11/12939-4, 12/03519-4, 15/12858-5, 11/18845-1)

AGUIAR, K. C. A.; MARSON, F. A. L.; GOMEZ, C. C. S.; PEREIRA, M. C.; PASCHOAL, I. A.; RIBEIRO, A. F.; RIBEIRO, J. D.. Physical performance, quality of life and sexual satisfaction evaluation in adults with cystic fibrosis: An: unexplored correlation. Revista Portuguesa de Pneumologia, v. 23, n. 4, p. 179-192, JUL-AUG 2017. (11/18845-1, 15/12183-8, 12/05458-2, 11/12939-4, 15/12858-5)

FARIA, A. G.; MARSON, F. A. L.; RIBEIRO, A. F.; RIBEIRO, J. D.. The correlation between age and sweat chloride levels in sweat tests. Revista Portuguesa de Pneumologia, v. 23, n. 4, p. 227-230, JUL-AUG 2017. (11/12939-4, 15/12183-8, 11/18845-1, 15/12858-5)

MARQUES FERREIRA, ADRIANA CAROLINA; LIMA MARSON, FERNANDO AUGUSTO; COHEN, MILENA ANTONELLI; BERTUZZO, CARMEN SILVIA; LEVY, CARLOS EMILIO; RIBEIRO, ANTONIO FERNANDO; RIBEIRO, JOSE DIRCEU. Hypertonic Saline as a Useful Tool for Sputum Induction and Pathogen Detection in Cystic Fibrosis. Lung, v. 195, n. 4, p. 431-439, AUG 2017. (11/12939-4, 15/12183-8, 11/18845-1, 15/12858-5)

FURLAN, LARISSA LAZZARINI; LIMA MARSON, FERNANDO AUGUSTO; RIBEIRO, JOSE DIRCEU; BERTUZZO, CARMEN SILVIA; SALOMAO JUNIOR, JOAO BATISTA; SILVA SOUZA, DOROTEIA ROSSI. IL8 gene as modifier of cystic fibrosis: unraveling the factors which influence clinical variability. Human Genetics, v. 135, n. 8, p. 881-894, AUG 2016. (11/12939-4, 13/19052-0, 11/18845-1, 15/12858-5)

FARIA, ALETHEA GUIMARAES; LIMA MARSON, FERNANDO AUGUSTO; DE SOUZA GOMEZ, CARLA CRISTINA; GONCALVES DE OLIVEIRA RIBEIRO, MARIA ANGELA; MORAIS, LUCAS BRIOSCHI; SERVIDONI, MARIA DE FATIMA; BERTUZZO, CARMEN SILVIA; SAKANO, EULALIA; GOTO, MAURA; PASCHOAL, ILMA APARECIDA; et al. Quality of sweat test (ST) based on the proportion of sweat sodium (Na) and sweat chloride (Cl) as diagnostic parameter of cystic fibrosis: are we on the right way?. DIAGNOSTIC PATHOLOGY, v. 11, OCT 26 2016. (11/12939-4, 11/18845-1, 15/12858-5)

PEREIRA, STEPHANIE V. N.; RIBEIRO, JOSE D.; BERTUZZO, CARMEN S.; MARSON, FERNANDO A. L.. Interaction among variants in the SLC gene family (SLC6A14, SLC26A9, SLC11A1, and SLC9A3) and CFTR mutations with clinical markers of cystic fibrosis. PEDIATRIC PULMONOLOGY, v. 53, n. 7, p. 888-900, JUL 2018. (11/12939-4, 15/12183-8, 11/18845-1, 15/12858-5)

DENTINI, PRISCILA; LIMA MARSON, FERNANDO AUGUSTO; BONADIA, LUCIANA CARDOSO; BERTUZZO, CARMEN SILVIA; RIBEIRO, ANTONIO FERNANDO; LEVY, CARLOS EMILIO; RIBEIRO, JOSE DIRCEU. Burkholderia cepacia complex in cystic fibrosis in a Brazilian reference center. MEDICAL MICROBIOLOGY AND IMMUNOLOGY, v. 206, n. 6, p. 447-461, DEC 2017. (11/12939-4, 15/12858-5, 15/12183-8, 11/18845-1)

GONCALVES BONALDO, TAISA CRESPI; SOUZA GOMEZ, CARLA CRISTINA; FRANCISCO PARAZZI, PALOMA LOPES; BRESCIANI ALMEIDA, CELIZE CRUZ; LIMA MARSON, FERNANDO AUGUSTO; DE OLIVEIRA, THEREZINHA; ALVES GRANDE, ROSANGELA APARECIDA; COHEN, MILENA ANTONELLI. Use of Non-Invasive Ventilation in Children and Adolescents: Scientific Evidence. CURRENT RESPIRATORY MEDICINE REVIEWS, v. 13, n. 2, p. 82-92, 2017. (11/12939-4, 15/12183-8, 11/18845-1, 15/12858-5)

LUIZ, LIVIA COCATO; LIMA MARSON, FERNANDO AUGUSTO; BRESCIANI ALMEIDA, CELIZE CRUZ; DALBO CONTRERA TORO, ADYLEIA APARECIDA; NUCCI, ANAMARLI; RIBEIRO, JOSE DIRCEU. Analysis of motor and respiratory function in Duchenne muscular dystrophy patients. Respiratory Physiology & Neurobiology, v. 262, p. 1-11, APR 2019. (11/12939-4, 15/12183-8, 11/18845-1, 15/12858-5)

ANDRESSA OLIVEIRA PEIXOTO; FERNANDO AUGUSTO LIMA MARSON; TIAGO HENRIQUE SOUZA; ANDREA DE MELO ALEXANDRE FRAGA; JOSÉ DIRCEU RIBEIRO. Lung ultrasound assessment of response to antibiotic therapy in cystic fibrosis exacerbations: a study of two cases. Jornal Brasileiro de Pneumologia, v. 45, n. 6, p. -, 2019. (11/12939-4, 15/12183-8, 11/18845-1, 15/12858-5)

SOUZA GOMEZ, CARLA CRISTINA; FRANCISCO PARAZZI, PALOMA LOPES; CLINCKSPOOR, KARL JAN; MAUCH, RENAN MARRICHI; TEIXEIRA PESSINE, FRANCISCO BENEDITO; LEVY, CARLOS EMILIO; PEIXOTO, ANDRESSA OLIVEIRA; GONCALVES OLIVEIRA RIBEIRO, MARIA ANGELA; RIBEIRO, ANTONIO FERNANDO; CONRAD, DOUGLAS; et al. Safety, Tolerability, and Effects of Sodium Bicarbonate Inhalation in Cystic Fibrosis. CLINICAL DRUG INVESTIGATION, v. 40, n. 2, p. 13-pg., 2019-11-13. (15/12858-5, 11/18845-1, 15/12183-8, 11/12939-4)

WOLLMEISTER, ELINARA; ALVAREZ, ALFONSO EDUARDO; SANTIAGO BASTOS, JULIANA CRISTINA; LIMA MARSON, FERNANDO AUGUSTO; RIBEIRO, JOSE DIRCEU; ELIAS BARACAT, EMILIO CARLOS; ARNS, CLARICE WEIS; LOPES RICCETTO, ADRIANA GUT. Respiratory syncytial virus in Brazilian infants - Ten years, two cohorts. Journal of Clinical Virology, v. 98, p. 33-36, JAN 2018. (11/12939-4, 15/12183-8, 11/18845-1, 15/12858-5)