| Full text | |
| Author(s): |
Larissa Lazzarini Furlan
[1]
;
José Dirceu Ribeiro
[2]
;
Carmen Sílvia Bertuzzo
[3]
;
João Batista Salomão Junior
;
Dorotéia Rossi Silva Souza
[5]
;
Fernando Augusto Lima Marson
Total Authors: 6
|
| Affiliation: | [1] Faculdade de Medicina de São José do Rio Preto - Brasil
[2] Universidade Estadual de Campinas. Faculdade de Ciências Médicas. Departamento de Pediatria - Brasil
[3] Universidade Estadual de Campinas. Faculdade de Ciências Médicas. Departamento de Genética Médica - Brasil
[5] Faculdade de Medicina de São José do Rio Preto. Centro de Pesquisa de Bioquímica e Biologia Molecular. Departamento de Biologia Molecular - Brasil
Total Affiliations: 6
|
| Document type: | Journal article |
| Source: | Jornal de Pediatria; v. 93, n. 6, p. 639-648, 2017-12-00. |
| Abstract | |
Abstract Objective: Interleukin 8 protein promotes inflammatory responses, even in airways. The presence of interleukin 8 gene variants causes altered inflammatory responses and possibly varied responses to inhaled bronchodilators. Thus, this study analyzed the interleukin 8 variants (rs4073, rs2227306, and rs2227307) and their association with the response to inhaled bronchodilators in cystic fibrosis patients. Methods: Analysis of interleukin 8 gene variants was performed by restriction fragment length polymorphism of polymerase chain reaction. The association between spirometry markers and the response to inhaled bronchodilators was evaluated by Mann-Whitney and Kruskal-Wallis tests. The analysis included all cystic fibrosis patients, and subsequently patients with two mutations in the cystic fibrosis transmembrane conductance regulator gene belonging to classes I to III. Results: This study included 186 cystic fibrosis patients. There was no association of the rs2227307 variant with the response to inhaled bronchodilators. The rs2227306 variant was associated with FEF50% in the dominant group and in the group with two identified mutations in the cystic fibrosis transmembrane conductance regulator gene. The rs4073 variant was associated with spirometry markers in four genetic models: co-dominant (FEF25-75% and FEF75%), dominant (FEV1, FEF50%, FEF75%, and FEF25-75%), recessive (FEF75% and FEF25-75%), and over-dominant (FEV1/FVC). Conclusions: This study highlighted the importance of the rs4073 variant of the interleukin 8 gene, regarding response to inhaled bronchodilators, and of the assessment of mutations in the cystic fibrosis transmembrane conductance regulator gene. (AU) | |
| FAPESP's process: | 13/19052-0 - IL-8 gene as a modifier of cystic fibrosis |
| Grantee: | Larissa Lazzarini Furlan |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |
| FAPESP's process: | 15/12858-5 - Identification of prevalent mutations and clinical and functional characterization of children and adults with primary ciliary dyskinesia |
| Grantee: | Fernando Augusto de Lima Marson |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 11/18845-1 - Association between polymorphisms in modifier genes in children and adolescent with allergic and non-allergic mild, moderate and severe asthma |
| Grantee: | Jose Dirceu Ribeiro |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 15/12183-8 - Identification of prevalent mutations and clinical and functional characterization of children and adults with primary ciliary dyskinesia |
| Grantee: | Jose Dirceu Ribeiro |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 11/12939-4 - Association between polymorphisms in modifier genes in children and adolescent with allergic and non-allergic: mild, moderate and severe asthma |
| Grantee: | Fernando Augusto de Lima Marson |
| Support Opportunities: | Scholarships in Brazil - Doctorate |