Variants in the interleukin 8 gene and the response to inhaled bronchodilators in cystic fibrosis

Autor(es)

Furlan, Larissa Lazzarini ^[1] ; Ribeiro, Jose Dirceu ^[2] ; Bertuzzo, Carmen Silvia ^[3] ; Salomao Junior, Joao Batista ^{[4, 5]} ; Silva Souza, Doroteia Rossi ^[6] ; Lima Marson, Fernando Augusto ^{[2, 3]}

Afiliação do(s) autor(es)

^[1] Fac Med Sao Jose do Rio Preto, Sao Jose Do Rio Preto, SP - Brazil

^[2] Univ Estadual Campinas UNICAMP, Fac Ciencias Med, Dept Pediat, Campinas, SP - Brazil

^[3] Univ Estadual Campinas Unicamp, Fac Ciencias Med, Dept Genet Med, Campinas, SP - Brazil

^[4] Hosp Univ, Fac Med Sao Jose Do Rio Preto, Dept Pediat, Sao Jose Do Rio Preto, SP - Brazil

^[5] Hosp Univ, Fac Med Sao Jose Do Rio Preto, Dept Pneumol Pediat, Sao Jose Do Rio Preto, SP - Brazil

^[6] Ctr Pesquisa Bioquim & Biol Mol, Fac Med Sao Jose Do Rio Preto, Dept Biol Mol, Sao Jose Do Rio Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Jornal de Pediatria; v. 93, n. 6, p. 639-648, NOV-DEC 2017.
• Citações Web of Science: 5

Resumo

Objective: Interleukin 8 protein promotes inflammatory responses, even in airways. The presence of interleukin 8 gene variants causes altered inflammatory responses and possibly varied responses to inhaled bronchodilators. Thus, this study analyzed the interleukin 8 variants (rs4073, rs2227306, and rs2227307) and their association with the response to inhaled bronchodilators in cystic fibrosis patients. Methods: Analysis of interleukin 8 gene variants was performed by restriction fragment length polymorphism of polymerase chain reaction. The association between spirometry markers and the response to inhaled bronchodilators was evaluated by Mann-Whitney and Kruskal-Wallis tests. The analysis included all cystic fibrosis patients, and subsequently patients with two mutations in the cystic fibrosis transmembrane conductance regulator gene belonging to classes I to III. Results: This study included 186 cystic fibrosis patients. There was no association of the rs2227307 variant with the response to inhaled bronchodilators. The rs2227306 variant was associated with FEF50% in the dominant group and in the group with two identified mutations in the cystic fibrosis transmembrane conductance regulator gene. The rs4073 variant was associated with spirometry markers in four genetic models: co-dominant (FEF25-75% and FEF75%), dominant (FEV1, FEF50%, FEF75%, and FEF25-75%), recessive (FEF75% and FEF25-75%), and over-dominant (FEV1/FVC). Conclusions: This study highlighted the importance of the rs4073 variant of the interleukin 8 gene, regarding response to inhaled bronchodilators, and of the assessment of mutations in the cystic fibrosis transmembrane conductance regulator gene. (C) 2017 Sociedade Brasileira de Pediatria. Published by Elsevier Editora Ltda. (AU)

• Processo FAPESP: 11/18845-1 - Associação entre polimorfismos de genes moduladores em crianças e adolescentes com asma alérgica e não alérgica leve, moderada e grave
• Beneficiário: Jose Dirceu Ribeiro
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 15/12183-8 - Identificação das mutações prevalentes e caracterização clínica e funcional de crianças e adultos com discinesia ciliar primária
• Beneficiário: Jose Dirceu Ribeiro
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/12939-4 - Associação entre polimorfismos de genes moduladores em crianças e adolescentes com asma alérgica e não alérgica: leve, moderada e grave
• Beneficiário: Fernando Augusto de Lima Marson
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 13/19052-0 - Gene IL-8 como modificador da fibrose cística
• Beneficiário: Larissa Lazzarini Furlan
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 15/12858-5 - Identificação das mutações prevalentes e caracterização clínica e funcional de crianças e adultos com discinesia ciliar primária
• Beneficiário: Fernando Augusto de Lima Marson
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado