Decaffeinated green tea extract rich in epigallocatechin-3-gallate improves insulin resistance and metabolic profiles in normolipidic diet-but not high-fat diet-fed mice

Autor(es):Mostrar menos -

Santana, Aline ^[1] ; Santamarina, Aline ^[1] ; Souza, Gabriel ^[2] ; Mennitti, Lais ^[3] ; Okuda, Marcos ^[1] ; Venancio, Daniel ^[4] ; Seelaender, Marilia ^[5] ; do Nascimento, Claudia Oiler ^[1] ; Ribeiro, Eliane ^[1] ; Lira, Fabio ^[6] ; Oyama, Lila ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Dept Fisiol, BR-04023062 Sao Paulo, SP - Brazil

^[2] Univ Sao Judas Tadeu, Lab Movimento Humano, Sao Paulo - Brazil

^[3] Univ Fed Sao Paulo, Programa Posgrad Interdisciplinar Ciencias Saude, BR-04023062 Sao Paulo, SP - Brazil

^[4] Univ Fed Sao Paulo, Dept Psicobiol, BR-04023062 Sao Paulo, SP - Brazil

^[5] Univ Fed Sao Paulo, Inst Biomed Sci, Canc Metab Res Grp, BR-04023062 Sao Paulo, SP - Brazil

^[6] Univ Estadual Paulista, Dept Phys Educ, Exercise & Immunometab Res Grp, Presidente Prudente, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF NUTRITIONAL BIOCHEMISTRY; v. 26, n. 9, p. 893-902, SEP 2015.
• Citações Web of Science: 12

Resumo

Supplementation with epigallocatechin-3-gallate (EGCG), which restores metabolic profiles, has been proposed as an option for preventing and treating obesity. We investigated whether decaffeinated green tea extract rich in EGCG, attenuates high-fat diet (HFD)-induced metabolic alterations in Swiss mice. The mice were maintained on either a control diet (CD) or HFD for 8 weeks and supplemented with either a placebo or EGCG (50 mg/kg/day). Body weight, serum lipid profiles, cytokine protein expression, and content in epididymal (EPI) and retroperitoneal (RET) adipose tissues, and adipocyte area were measured. The body weights of HFD + placebo-fed mice were increased compared with those of HFD + EGCG-fed mice (28 and 21%, respectively), whereas the body weights of CD + EGCG-fed mice were decreased 16% compared with those of the CD + placebo group. Serum triglyceride levels were decreased 32% in the CD + EGCG group compared with the CD + placebo group. Compared with the CD + placebo group, increased phosphorylation of AMPK and hormone-sensitive lipase in EPI and RET, respectively, was found in the CD + EGCG group. Increased acetyl-CoA carboxylase phosphorylation was observed in both adipose tissues. In addition, TNF-alpha and IL-10 levels in EPI and adiponectin levels were higher in the CD + EGCG group than in the CD + placebo group. TNF-alpha levels were lower in the HFD + EGCG group than in the HFD + placebo group. Furthermore, the CD + EGCG group exhibited a lower adipocyte area than the CD + placebo group. These indicate that the effects of decaffeinated green tea extract on body mass may be related to the crosstalk between lipolytic and inflammatory pathways in normolipidic diet-fed mice but not in HFD-fed mice. (C) 2015 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 12/03713-5 - Efeito da ingestão de um extrato de chá verde rico em epigalocatequina-3-galato na expressão de citocinas e reguladores metabólicos no tecido adiposo e músculo esquelético de camundongos obesos induzidos por dieta hiperlipídica
• Beneficiário: Lila Missae Oyama
• Modalidade de apoio: Auxílio à Pesquisa - Regular