Long-Term Therapy With Omega-3 Ameliorates Myonecrosis and Benefits Skeletal Muscle Regeneration in Mdx Mice

Autor(es)

Apolinario, Leticia Montanholi ^[1] ; De Carvalho, Samara Camacari ^[1] ; Neto, Humberto Santo ^[1] ; Marques, Maria Julia ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, UNICAMP, Inst Biol, Dept Biol Estrutural & Func, BR-1083970 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Anatomical Record-Advances in Integrative Anatomy and Evolutionary Biology; v. 298, n. 9, p. 1589-1596, SEP 2015.
• Citações Web of Science: 10

Resumo

In Duchenne muscle dystrophy (DMD) and in the mdx mouse model of DMD, a lack of dystrophin leads to myonecrosis and cardiorespiratory failure. Several lines of evidence suggest a detrimental role of the inflammatory process in the dystrophic process. Previously, we demonstrated that short-term therapy with eicosapentaenoic acid (EPA), at early stages of disease, ameliorated dystrophy progression in the mdx mouse. In the present study, we evaluated the effects of a long-term therapy with omega-3 later in dystrophy progression. Three-month-old mdx mice received omega-3 (300 mg/kg) or vehicle by gavage for 5 months. The quadriceps and diaphragm muscles were removed and processed for histopathology and Western blot. Long-term therapy with omega-3 increased the regulatory protein MyoD and muscle regeneration and reduced markers of inflammation (TNF-alpha and NF-kB) in both muscles studied. The present study supports the long-term use of omega-3 at later stages of dystrophy as a promising option to be investigated in DMD clinical trials. (C) 2015 Wiley Periodicals, Inc. (AU)

• Processo FAPESP: 10/14750-3 - Ácido eicosapentanóico x deflazacorte: mecanismos de ação e comparação de efeitos em camundongos distróficos mdx
• Beneficiário: Letícia Montanholi Apolinário
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 08/58491-1 - Mecanismos de proteção da distrofia muscular: estudo proteômico e terapia farmacológica
• Beneficiário: Maria Julia Marques
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/51697-6 - Mecanismos de acao do acido eicosapentanoico (epa) na protecao a mionecrose em fibras musculares distroficas
• Beneficiário: Maria Julia Marques
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/02474-4 - Tratamento in vivo e in vitro com bloqueador de cálcio e antioxidante em camundongos mdx
• Beneficiário: Elaine Minatel
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 10/13412-7 - População de macrófagos em músculos esqueléticos de camundongos mdx tratados com ácido eicosapentanóico
• Beneficiário: Samara Camaçarí de Carvalho
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 14/04782-6 - Estudos pré-clínicos no camundongo mdx: metabolômica, biomarcadores e terapia com ômega-3
• Beneficiário: Maria Julia Marques
• Modalidade de apoio: Auxílio à Pesquisa - Regular