Unified chromatography: Fundamentals, instrumentation and applications

Autor(es)

Silva, Meire R. ^[1] ; Andrade, Felipe N. ^[1] ; Fumes, Bruno H. ^[1] ; Lancas, Fernando M. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Chem Sao Carlos, BR-13560970 Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF SEPARATION SCIENCE; v. 38, n. 17, p. 3071-3083, SEP 2015.
• Citações Web of Science: 5

Resumo

The concept of unified chromatography has been in existence for 50 years after the work of Giddings proposing that all modes of chromatography (gas chromatography, liquid chromatography, supercritical fluid chromatography and so on) may be treated together under a single unified theory. His idea was partially fulfilled 23 years later by Ishii, Takeuchi and colleagues, who demonstrated for the first time the possibility to analyze a complex sample containing substances with a wide range of boiling points and polarities in the same instrument and column, just by varying the mobile phase pressure and temperature to change from one chromatographic mode to another. This approach has been demonstrated through application to the separation of complex mixtures in several areas including crude oil, edible oils and polymers. Still, unified chromatography has not yet been fully developed. In the present work, we will review the fundamentals, instrumentation and several applications of the technique. Also discussed are the drawbacks that still hinder development, as well as the recent developments and trends in instrumentation and columns that suggest the most feasible ways forward to the full development of unified chromatography. (AU)

• Processo FAPESP: 12/22055-9 - Desenvolvimento de sílica superficialmente modificada com líquidos iônicos e seu uso em técnicas de preparo de amostra
• Beneficiário: Meire Ribeiro da Silva
• Linha de fomento: Bolsas no Brasil - Doutorado
• Processo FAPESP: 14/07347-9 - Desenvolvimento de novos materiais e dispositivos para técnicas miniaturizadas de preparo de amostras na área de segurança alimentar
• Beneficiário: Fernando Mauro Lanças
• Linha de fomento: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/09898-4 - Desenvolvimento de polímeros molecularmente impressos e seu uso para o preparo de amostras e análise de triazinas e sulfoniluréias em milho utilizando cromatografia líquida de alta eficiência (HPLC).
• Beneficiário: Felipe Nascimento Andrade
• Linha de fomento: Bolsas no Brasil - Doutorado
• Processo FAPESP: 13/17425-4 - Avaliação do emprego da técnica MEPS na análise de agrotóxicos em caldo de cana-de-açúcar por GC-MS.
• Beneficiário: Bruno Henrique Fumes
• Linha de fomento: Bolsas no Brasil - Mestrado