Acetylcholinesterase immobilized on modified magnetic beads as a tool for screening a compound library

Autor(es)

Vanzolini, Kenia L. ^[1] ; Vieira, Lucas C. C. ^[1] ; Correa, Arlene. G. ^[1] ; Moaddel, Ruin ^[2] ; Cass, Quezia B. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Carlos, Dept Quim, SEPARARE Nucleo Pesquisa Cromatog, BR-13565905 Sao Carlos, SP - Brazil

^[2] NIA, Biomed Res Ctr, NIH, Baltimore, MD 21224 - USA

• Tipo de documento: Artigo Científico
• Fonte: Microchimica Acta; v. 182, n. 13-14, p. 2209-2213, OCT 2015.
• Citações Web of Science: 8

Resumo

Acetylcholinesterase (AChE) from Electrophorus electricus was immobilized on the surface of amino-modified magnetic beads (AChE-MB), and its activity evaluated by the quantification of acetylcholine hydrolysis. A reference mixture composed of AChE binders (galanthamine and a probe coumarin, K (i) = 0.031 +/- 0.010 mu M) and non-binders (ketamine and propranolol) was used to probe the fishing assay. The performance of the bioconjugation assay was demonstrated with a library of 12 reference coumarins from which two ligands were directly identified by LC-MS/MS in a single assay, demonstrating the usefulness of this approach. (AU)

• Processo FAPESP: 13/02054-0 - Enzimas imobilizadas: novos modelos para a triagem de ligantes
• Beneficiário: Kenia Lourenço Vanzolini
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 13/01710-1 - Ligantes enzimáticos: novos modelos de triagem
• Beneficiário: Quezia Bezerra Cass
• Modalidade de apoio: Auxílio à Pesquisa - Temático