| Full text | |
| Author(s): |
Vanzolini, Kenia L.
[1]
;
Vieira, Lucas C. C.
[1]
;
Correa, Arlene. G.
[1]
;
Moaddel, Ruin
[2]
;
Cass, Quezia B.
[1]
Total Authors: 5
|
| Affiliation: | [1] Univ Fed Sao Carlos, Dept Quim, SEPARARE Nucleo Pesquisa Cromatog, BR-13565905 Sao Carlos, SP - Brazil
[2] NIA, Biomed Res Ctr, NIH, Baltimore, MD 21224 - USA
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | Microchimica Acta; v. 182, n. 13-14, p. 2209-2213, OCT 2015. |
| Web of Science Citations: | 8 |
| Abstract | |
Acetylcholinesterase (AChE) from Electrophorus electricus was immobilized on the surface of amino-modified magnetic beads (AChE-MB), and its activity evaluated by the quantification of acetylcholine hydrolysis. A reference mixture composed of AChE binders (galanthamine and a probe coumarin, K (i) = 0.031 +/- 0.010 mu M) and non-binders (ketamine and propranolol) was used to probe the fishing assay. The performance of the bioconjugation assay was demonstrated with a library of 12 reference coumarins from which two ligands were directly identified by LC-MS/MS in a single assay, demonstrating the usefulness of this approach. (AU) | |
| FAPESP's process: | 13/02054-0 - Enzyme immobilization: new tools for ligand screening |
| Grantee: | Kenia Lourenço Vanzolini |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 13/01710-1 - Enzyme ligand: new models of screening |
| Grantee: | Quezia Bezerra Cass |
| Support Opportunities: | Research Projects - Thematic Grants |