| Texto completo | |
| Autor(es): Mostrar menos - |
Carlos, Daniela
[1, 2]
;
Yaochite, Juliana N. U.
[1, 2]
;
Rocha, Fernanda A.
[1, 2]
;
Toso, Vanina D.
[3]
;
Malmegrim, Kelen C. R.
[4]
;
Ramos, Simone G.
[5]
;
Jamur, Maria C.
[3]
;
Oliver, Constance
[3]
;
Camara, Niels O.
[6]
;
Andrade, Marcus V. M.
[7]
;
Cunha, Fernando Q.
[8]
;
Silva, Joao S.
[1, 2]
Número total de Autores: 12
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Sch Med Ribeirao Preto, Dept Biochem, BR-14049900 Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Sch Med Ribeirao Preto, Dept Immunol, BR-14049900 Ribeirao Preto, SP - Brazil
[3] Univ Sao Paulo, Sch Med Ribeirao Preto, Mol & Cellular Biol, BR-14049900 Ribeirao Preto, SP - Brazil
[4] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Dept Clin Toxicol & Bromatol Anal, BR-14049900 Ribeirao Preto, SP - Brazil
[5] Univ Sao Paulo, Sch Med Ribeirao Preto, Pathol, BR-14049900 Ribeirao Preto, SP - Brazil
[6] Univ Sao Paulo, Inst Biomed Sci ICB, Dept Immunol, Sao Paulo, SP - Brazil
[7] Univ Fed Minas Gerais, Sch Med, Dept Clin Med, Belo Horizonte, MG - Brazil
[8] Univ Sao Paulo, Sch Med Ribeirao Preto, Pharmacol, BR-14049900 Ribeirao Preto, SP - Brazil
Número total de Afiliações: 8
|
| Tipo de documento: | Artigo Científico |
| Fonte: | European Journal of Immunology; v. 45, n. 10, p. 2873-2885, OCT 2015. |
| Citações Web of Science: | 10 |
| Resumo | |
Quantitative alterations in mast cell numbers in pancreatic lymph nodes (PLNs) have been reported to be associated with type 1 diabetes (T1D) progression, but their potential role during T1D remains unclear. In this study, we evaluated the role of mast cells in T1D induced by multiple low-dose streptozotocin (MLD-STZ) treatments, using two strains of mast cell-deficient mice (W/W-v or Wsh/Wsh) and the adoptive transfer of mast cells. Mast cell deficient mice developed severe insulitis and accelerated hyperglycemia, with 100% of mice becoming diabetic compared to their littermates. In parallel, these diabetic mice had decreased numbers of T regulatory (Treg) cells in the PLNs. Additionally, mast cell deficiency caused a significant reduction in IL-10, TGF-, and IL-6 expression in the pancreatic tissue. Interestingly, IL-6-deficient mice are more susceptible to T1D associated with reduced Treg-cell numbers in the PLNs, but mast cell transfer from wild-type mice induced protection to T1D in these mice. Finally, mast cell adoptive transfer prior to MLD-STZ administration conferred resistance to T1D, promoted increased Treg cells, and decreased IL-17-producing T cells in the PLNs. Taken together, our results indicate that mast cells are implicated in resistance to STZ-induced T1D via an immunological tolerance mechanism mediated by Treg cells. (AU) | |
| Processo FAPESP: | 08/50598-1 - Papel dos mastócitos na diabetes experimental e no aumento de suscetibilidade ao desenvolvimento da sepse |
| Beneficiário: | Daniela Carlos Sartori |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |