Role of protein kinase C in cytokine secretion by lung epithelial cells during infection with Paracoccidioides brasiliensis

Autor(es)

Alcantara, Cristiane ^[1] ; Maza, Paloma Korehisa ^[1] ; Silva, Bianca Carla ^[1] ; Barros, Campitelli ^[1] ; Suzuki, Erika ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, BR-04023062 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PATHOGENS AND DISEASE; v. 73, n. 7 OCT 2015.
• Citações Web of Science: 2

Resumo

In this study, we investigated the role of protein kinases C (PKCs) in interleukin (IL)-6 and IL-8 secretion by human lung epithelial A549 cells during infection with the fungal pathogen Paracoccidioides brasiliensis. Rottlerin and the broad spectrum PKC inhibitor Go 6983 reduced cytokine levels in A549 cell-P. brasiliensis cultures. Next, by western blot, we verified that infection with this fungus led to phosphorylation of PKC delta (Thr(505)). By using a peptide inhibitor for PKC delta or PKC delta short interfering RNA technique, IL-6 and IL-8 levels in A549-P. brasiliensis cultures were also reduced. Together, these results indicate that P. brasiliensis promotes IL-6 and IL-8 secretion by A549 cells in a PKC delta-dependent manner. (AU)

• Processo FAPESP: 11/22773-6 - Estudos sobre os mecanismos de adesão e modulação de citocinas durante a interação entre células epiteliais e fungos patogênicos
• Beneficiário: Erika Suzuki de Toledo
• Modalidade de apoio: Auxílio à Pesquisa - Regular