Role of protein kinase C in cytokine secretion by lung epithelial cells during infection with Paracoccidioides brasiliensis

Author(s)

Alcantara, Cristiane ^[1] ; Maza, Paloma Korehisa ^[1] ; Silva, Bianca Carla ^[1] ; Barros, Campitelli ^[1] ; Suzuki, Erika ^[1]

Affiliation

^[1] Univ Fed Sao Paulo, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, BR-04023062 Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: PATHOGENS AND DISEASE; v. 73, n. 7 OCT 2015.
• Web of Science Citations: 2

Abstract

In this study, we investigated the role of protein kinases C (PKCs) in interleukin (IL)-6 and IL-8 secretion by human lung epithelial A549 cells during infection with the fungal pathogen Paracoccidioides brasiliensis. Rottlerin and the broad spectrum PKC inhibitor Go 6983 reduced cytokine levels in A549 cell-P. brasiliensis cultures. Next, by western blot, we verified that infection with this fungus led to phosphorylation of PKC delta (Thr(505)). By using a peptide inhibitor for PKC delta or PKC delta short interfering RNA technique, IL-6 and IL-8 levels in A549-P. brasiliensis cultures were also reduced. Together, these results indicate that P. brasiliensis promotes IL-6 and IL-8 secretion by A549 cells in a PKC delta-dependent manner. (AU)

• FAPESP's process: 11/22773-6 - Studies of the mechanisms of adhesion and cytokine modulation during interaction of epithelial cells and pathogenic fungi
• Grantee: Erika Suzuki de Toledo
• Support Opportunities: Regular Research Grants