Dependence of cytokine levels on the sex of experimental animals: a pilot study on the effect of oestrogen in the temporomandibular joint synovial tissues

Autor(es)

Figueroba, S. R. ^[1] ; Franco, G. C. N. ^[2] ; Omar, N. F. ^[3] ; Groppo, M. F. ^[3] ; Groppo, F. C. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, UNICAMP, Piracicaba Dent Sch, Dept Physiol Sci, BR-13414903 Sao Paulo - Brazil

^[2] Univ Estadual Ponta Grossa, Dept Gen Biol, Area Physiol, Pathophysiol, Ponta Grossa, Parana - Brazil

^[3] Univ Estadual Campinas, UNICAMP, Piracicaba Dent Sch, Dept Morphol, BR-13414903 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: International Journal of Oral and Maxillofacial Surgery; v. 44, n. 11, p. 1368-1375, NOV 2015.
• Citações Web of Science: 4

Resumo

The aim of this study was to evaluate the effects of 17 beta-oestradiol (E2) on cartilage thickness and cytokine levels in the temporomandibular joint (TMJ). Thirty rats (15 female, 15 male) were orchidectomized (ORX), ovariectomized (OVX), or sham-operated. After 21 days, animals were assigned to six groups: (1) sham-ORX; (2) ORX; (3) ORX+E2; (4) sham-OVX; (5) OVX; and (6) OVX+E2. Treatments were administered daily for 21 days. The thickness of cartilage layers (fibrous, proliferative, maturation, and hypertrophic) and cytokine levels (interleukins IL-1 alpha, IL-1 beta, IL-6, and tumour necrosis factor alpha (TNF-alpha)) were measured by histomorphometry and ELISA, respectively. Kruskal-Wallis/Durm's tests were used (alpha = 5%). Sham-ORX showed thicker layers than ORX+E2, but not thicker than ORX. All layers, except the hypertrophic layer, were thicker in sham-OVX than OVX or OVX+E2. Although IL-1 beta levels were higher in castrated animals, E2 did not affect the level of this cytokine. IL-1 alpha levels were higher in both ORX (P = 0.0010) and ORX+E2 (P = 0.0053) than in sham-ORX. However, E2 decreased IL-1 alpha levels in OVX (P = 0.0129). When compared to sham-ORX/OVX, IL-6 levels were not affected by E2 in males but were reduced in OVX (P = 0.0079) and increased in OVX+E2 (P = 0.0434). Levels of TNF-alpha were reduced by E2 in both ORX+E2 and OVX+E2. E2 treatment caused gender- and layer-dependent changes in the cartilage. Castration increased all cytokine levels, except for IL-6, without respect to gender. (AU)

• Processo FAPESP: 06/05023-5 - Influência da reposição hormonal e de antiinflamatórios sobre parâmetros morfológicos, histoquímicos e imunohistoquímicos em ratos com ou sem DTM induzida
• Beneficiário: Francisco Carlos Groppo
• Modalidade de apoio: Auxílio à Pesquisa - Regular