Root canal content from primary endodontic infection and upregulation of gelatinases in fibroblast cells

Autor(es)

Herrera, D. R. ^[1] ; Silva, E. J. N. L. ^{[1, 2]} ; Santos, C. C. ^[1] ; Zaia, A. A. ^[1] ; Ferraz, C. C. R. ^[1] ; Almeida, J. F. A. ^[1] ; Gomes, B. P. F. A. ^[1]

Afiliação do(s) autor(es)

^[1] State Univ Campinas UNICAMP, Piracicaba Dent Sch, Dept Restorat Dent, Endodont Div, BR-13414903 Sao Paulo - Brazil

^[2] Grande Rio Univ UNIGRANRIO, Hlth & Sci Ctr, Rio De Janeiro - Brazil

• Tipo de documento: Artigo Científico
• Fonte: International Endodontic Journal; v. 48, n. 12, p. 1168-1174, DEC 2015.
• Citações Web of Science: 5

Resumo

AimTo investigate endotoxin levels from primary endodontic infections before and after chemomechanical preparation (CMP) and to determine their antigenicity against 3T3 fibroblasts through gelatinolytic activity of matrix metalloproteinases (MMPs). MethodologyTwenty-four root canals with primary endodontic infection and apical periodontitis were selected. Samples were collected using paper points before (S1) and after chemomechanical preparation (CMP) (S2). The limulus amebocyte lysate assay was used for endotoxin measurement. Fibroblasts were stimulated with root canal contents for 24h. Supernatants of cell cultures stimulated with root canal contents were collected after 24h to determine the levels of MMP-2 and MMP-9 gelatinolytic activity using the zymography technique. Friedman and Wilcoxon tests were used to compare the amount of endotoxin before (S1) and after CMP (S2) (P<0.05). Data obtained from gelatinolytic activity were analysed using anova and Tukey's tests (P<0.05). ResultsEndotoxin was recovered in 100% of the samples. There was a significant reduction in endotoxin levels after CMP (P<0.05). A correlation was found between the levels of endotoxins and MMP-2 expression (P<0.05). Root canal contents of initial samples (S1) induced significantly greater MMP-2 expression by fibroblasts when compared to S2 and the nonstimulated group (P<0.05). No gelatinolytic activity of MMP-9 was observed in S1, S2 and control group. ConclusionsRoot canal contents from primary endodontic infections had gelatinolytic activity for MMP-2. Moreover, CMP was effective in reducing endotoxin levels and their antigenicity against fibroblasts on gelatinolytic activity. (AU)

• Processo FAPESP: 11/50510-0 - Avaliação in vivo do efeito do preparo químico-mecânico sobre microrganismos e endotoxinas em infecções endodônticas persistentes e suscetibilidade antimicrobiana dos Enterococcus spp. isolados
• Beneficiário: Brenda Paula Figueiredo de Almeida Gomes
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 10/19136-1 - Estudo microbiológico e de endotoxinas em canais radiculares com necrose pulpar e lesão periapical e avaliação do seu potencial antigênico contra macrófagos na produção de IL1-beta e TNF-alfa durante a terapia endodôntica
• Beneficiário: Brenda Paula Figueiredo de Almeida Gomes
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 10/17877-4 - Análise microbiológica, de endoxinas e de citocinas pró-inflamatórias em infecções endodônticas primárias com presença de lesões periapicais
• Beneficiário: Brenda Paula Figueiredo de Almeida Gomes
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 10/13498-9 - Análise microbiológica, de endoxinas e de citocinas pró-inflamatórias em infecções endodônticas primárias com presença de lesões periapicais
• Beneficiário: Daniel Rodrigo Herrera Morante
• Modalidade de apoio: Bolsas no Brasil - Doutorado