Differential expression of lactic acid isomers, extracellular matrix metalloproteinase inducer, and matrix metalloproteinase-8 in vaginal fluid from women with vaginal disorders

Autor(es)

Beghini, J. ^{[1, 2]} ; Linhares, I. M. ^{[1, 3]} ; Giraldo, P. C. ^[2] ; Ledger, W. J. ^[1] ; Witkin, S. S. ^[1]

Afiliação do(s) autor(es)

^[1] Weill Cornell Med Coll, Dept Obstet & Gynecol, Div Immunol & Infect Dis, New York, NY 10065 - USA

^[2] Univ Estadual Campinas, Dept Gynecol & Obstet, Campinas, SP - Brazil

^[3] Univ Sao Paulo, Sch Med, Dept Gynecol & Obstet, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BJOG-AN INTERNATIONAL JOURNAL OF OBSTETRICS AND GYNAECOLOGY; v. 122, n. 12, p. 1580-1585, NOV 2015.
• Citações Web of Science: 12

Resumo

ObjectiveDo metabolites in vaginal samples vary between women with different vaginal disorders. DesignCross-sectional study. SettingCampinas, Brazil. SampleSeventy-seven women (39.9%) with no vaginal disorder, 52 women (26.9%) with vulvovaginal candidiasis (VVC), 43 women (22.3%) with bacterial vaginosis (BV), and 21 women (10.9%) with cytolytic vaginosis (CTV). MethodConcentrations of d- and l-lactic acid, extracellular matrix metalloproteinase inducer (EMMPRIN), and matrix metalloproteinase-8 (MMP-8), and the influence of Candida albicans on EMMPRIN production by cultured vaginal epithelial cells, were determined by enzyme-linked immunosorbent assay (ELISA). Associations were determined by the Mann-Whitney U-test and by Spearman's rank correlation test. Main outcome measuresMetabolite levels and their correlation with diagnoses. ResultsVaginal concentrations of d- and l-lactic acid were reduced from control levels in BV (P<0.0001); l-lactic acid levels were elevated in CTV (P=0.0116). EMMPRIN and MMP-8 concentrations were elevated in VVC (P<0.0001). EMMPRIN and l-lactic acid concentrations (P0.008), but not EMMPRIN and d-lactic acid, were correlated in all groups. EMMPRIN also increased in proportion with the ratio of l- to d-lactic acid in controls and in women with BV (P0.009). Concentrations of EMMPRIN and MMP-8 were correlated in controls and women with VVC (P0.0002). Candida albicans induced EMMPRIN release from vaginal epithelial cells. ConclusionsVaginal secretions from women with BV are deficient in d- and l-lactic acid, women with VVC have elevated EMMPRIN and MMP-8 levels, and women with CTV have elevated l-lactic acid levels. These deviations may contribute to the clinical signs, symptoms, and sequelae that are characteristic of these disorders. (AU)

• Processo FAPESP: 11/19960-9 - Resposta humoral cérvico-vaginal (IgA secretora) em mulheres que receberam a vacina quadrivalente contra o HPV
• Beneficiário: Paulo César Giraldo
• Modalidade de apoio: Auxílio à Pesquisa - Regular