Characterization of a two-peptide plantaricin produced by Lactobacillus plantarum MBSa4 isolated from Brazilian salami

Autor(es)

Barbosa, M. S. ^{[1, 2]} ; Todorov, S. D. ^[1] ; Ivanova, I. V. ^{[3, 2]} ; Belguesmia, Y. ^[2] ; Choiset, Y. ^[2] ; Rabesona, H. ^[2] ; Chobert, J. -M. ^[2] ; Haertle, T. ^[2] ; Franco, B. D. G. M. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Pharmaceut Sci, Dept Food & Expt Nutr, Sao Paulo, SP - Brazil

^[2] INRA, UR Biopolymeres Interact Assemblages 1268, Equipe Fonct & Interact Prot, F-44316 Nantes 3 - France

^[3] Univ Sofia, Fac Biol Sci, Dept Microbiol, BU-1126 Sofia - Bulgaria

• Tipo de documento: Artigo Científico
• Fonte: FOOD CONTROL; v. 60, p. 103-112, FEB 2016.
• Citações Web of Science: 13

Resumo

The aim of this study was to explore the biochemical and genetic features of the two-peptide bacteriocin produced by a Lactobacillus plantarum strain isolated from Italian type salami produced in Brazil (Lb. plantarum MBSa4). Identification of bacteriocinogenic Lb. plantarum MBSa4 was performed by 16S rRNA sequencing. Expressed bacteriocin was evaluated for spectrum of activity, heat and pH stability, mechanism of action, and molecular mass. Partial purification was achieved by cation-exchange, and reversed phase - HPLC. Total DNA of Lb. plantarum MBSa4 was extracted and tested for presence of previously described bacteriocin genes. Bacteriocin MBSa4 was heat-stable, unaffected by pH 2.0 to 6.0 and active against all tested Listeria monocytogenes strains and most of tested fungi. Maximal production (1600 AU/ml) in MRS broth occurred after 22 h at 25 degrees C, presenting bacteriostatic activity as result of combined action of two components. The molecular mass determined by SDS-PAGE was 23 kDa. PCR-amplified DNA indicated the same nucleotide sequence of plantaricin W. Results indicate that Lb. plantarum MBSa4 produces plantaricin W, a two-peptide lantibiotic with remarkable anti-Listeria activity. (c) 2015 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 08/58841-2 - Bacterias laticas produtoras de bacteriocinas em salame: isolamento, caracterizacao, encapsulacao e aplicacao no controle de listeria monocytogenes em salame
• Beneficiário: Matheus de Souza Barbosa
• Modalidade de apoio: Bolsas no Brasil - Doutorado