Camphene isolated from essential oil of Piper cernuum (Piperaceae) induces intrinsic apoptosis in melanoma cells and displays antitumor activity in vivo

Autor(es):Mostrar menos -

Girola, Natalia ^[1] ; Figueiredo, Carlos R. ^[1] ; Farias, Camyla F. ^[1] ; Azevedo, Ricardo A. ^[2] ; Ferreira, Adilson K. ^[2] ; Teixeira, Sarah F. ^[2] ; Capello, Tabata M. ^[3] ; Martins, Euder G. A. ^[4] ; Matsuo, Alisson L. ^[5] ; Travassos, Luiz R. ^[1] ; Lago, Joao H. G. ^[3]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Expt Oncol Unit UNONEX, BR-04023062 Sao Paulo, SP - Brazil

^[2] Univ Fed Sao Paulo, Lab Tumor Immunol, Inst Biomed Sci, BR-04023062 Sao Paulo, SP - Brazil

^[3] Univ Fed Sao Paulo, Inst Environm Chem & Pharmaceut Sci, BR-04023062 Sao Paulo, SP - Brazil

^[4] Univ Sao Paulo, Inst Biosci, BR-05508 Sao Paulo, SP - Brazil

^[5] Univ Fed Sao Paulo, Interdept Grp Hlth Econ Grides, BR-04023062 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Biochemical and Biophysical Research Communications; v. 467, n. 4, p. 928-934, NOV 27 2015.
• Citações Web of Science: 17

Resumo

Natural monoterpenes were isolated from the essential oil of Piper cernuum yell. (Piperaceae) leaves. The crude oil and the individual monoterpenes were tested for cytotoxicity in human tumor cell lineages and B16F10-Nex2 murine melanoma cells. In the present work we demonstrate the activity of camphene against different cancer cells, with its mechanism of action being investigated in vitro and in vivo in murine melanoma. Camphene induced apoptosis by the intrinsic pathway in melanoma cells mainly by causing endoplasmic reticulum (ER) stress, with release of Ca2+ together with HmgB1 and calreticulin, loss of mitochondrial membrane potential and up regulation of caspase-3 activity. Importantly, camphene exerted antitumor activity in vivo by inhibiting subcutaneous tumor growth of highly aggressive melanoma cells in a syngeneic model, suggesting a promising role of this compound in cancer therapy. (C) 2015 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 11/51739-0 - Uso sustentável da biodiversidade de áreas remanescentes da Mata Atlântica do Estado de São Paulo: avaliação, isolamento e caracterização molecular de metabólitos secundários bioativos em espécies vegetais
• Beneficiário: João Henrique Ghilardi Lago
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Regular
• Processo FAPESP: 15/11936-2 - Emprego da quimiodiversidade de espécies vegetais de áreas remanescentes da Mata Atlântica do Estado de São Paulo na seleção de protótipos biologicamente ativos
• Beneficiário: João Henrique Ghilardi Lago
• Modalidade de apoio: Auxílio à Pesquisa - Regular