Evolution in miniaturized column liquid chromatography instrumentation and applications: An overview

Autor(es)

Nazario, Carlos E. D. ^[1] ; Silva, Meire R. ^[2] ; Franco, Maraissa S. ^[2] ; Lancas, Fernando M. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Fed Goias, Dept Chem, Jatai, Go - Brazil

^[2] Univ Sao Paulo, Inst Chem Sao Carlos, BR-13560970 Sao Carlos, SP - Brazil

• Tipo de documento: Artigo de Revisão
• Fonte: Journal of Chromatography A; v. 1421, p. 18-37, NOV 20 2015.
• Citações Web of Science: 35

Resumo

The purpose of this article is to underline the miniaturized LC instrumental system and describe the evolution of commercially available systems by discussing their advantages and drawbacks. Nowadays, there are already many miniaturized LC systems available with a great variety of pump design, interface and detectors as well as efficient columns technologies and reduced connections devices. The solvent delivery systems are able to drive the mobile phase without flow splitters and promote gradient elution using either dual piston reciprocating or syringe-type pumps. The mass spectrometry as detection system is the most widely used detection system; among many alternative ionization sources direct-EI LC-MS is a promising alternative to APCI. In addition, capillary columns are now available showing many possibilities Of stationary phases, inner diameters and hardware materials. This review provides a discussion about miniaturized LC demonstrating fundamentals and instrumentals' aspects of the commercially available miniaturized LC instrumental system mainly nano and micro LC formats. This review also covers the recent developments and trends in instrumentation, capillary and nano columns, and several applications of this very important and promising field. (C) 2015 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 12/22055-9 - Desenvolvimento de sílica superficialmente modificada com líquidos iônicos e seu uso em técnicas de preparo de amostra
• Beneficiário: Meire Ribeiro da Silva
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 14/07347-9 - Desenvolvimento de novos materiais e dispositivos para técnicas miniaturizadas de preparo de amostras na área de segurança alimentar
• Beneficiário: Fernando Mauro Lanças
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 05/59360-0 - Miniaturização de instrumentação analítica para técnicas de separação: do preparo de amostra a impressão do resultado
• Beneficiário: Fernando Mauro Lanças
• Modalidade de apoio: Auxílio à Pesquisa - Temático