Characterization of allele-specific expression of the X-linked gene MAO-A in trophectoderm cells of bovine embryos produced by somatic cell nuclear transfer

Autor(es)

Ferreira, A. R. ^{[1, 2]} ; Aguiar Filho, L. F. C. ^[2] ; Sousa, R. V. ^[2] ; Sartori, R. ^{[3, 1]} ; Franco, M. M. ^{[4, 5, 2]}

Afiliação do(s) autor(es)

^[1] Univ Estadual Sao Paulo Julio de Mesquita Filho, Escola Med Vet & Zootecnia, Dept Reprod Anim & Radiol Vet, Botucatu, SP - Brazil

^[2] Embrapa Recursos Genet & Biotecnol, Lab Reprod Anim, Brasilia, DF - Brazil

^[3] Univ Sao Paulo, Escola Super Agr Luiz de Queiroz, Dept Zootecnia, Piracicaba, SP - Brazil

^[4] Univ Fed Uberlandia, Inst Genet & Bioquim, BR-38400 Uberlandia, MG - Brazil

^[5] Univ Fed Uberlandia, Fac Med Vet, BR-38400 Uberlandia, MG - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Genetics and Molecular Research; v. 14, n. 4, p. 12128-12136, 2015.
• Citações Web of Science: 1

Resumo

Somatic cell nuclear transfer (SCNT) may affect epigenetic mechanisms and alter the expression of genes related to embryo development and X chromosome inactivation (XCI). We characterized allele-specific expression of the X-linked gene monoamine oxidase type A (MAO-A) in the trophectoderm (TF) of embryos produced by SCNT. Total RNA was isolated from individual biopsies (N = 25), and the allele-specific expression assessed by reverse transcription-polymerase chain reaction-restriction fragment length polymorphism. Both paternal and maternal alleles were expressed in the trophectoderm. However, a higher frequency of the mono-allelic expression of a specific allele was observed (N = 17; 68%), with the remaining samples showing the presence of mRNA from both alleles (N = 8; 32%). Considering that MAO-A is subject to XCI in bovine, our results suggest that SCNT may influence XCI because neither an imprinted (mono-allelic expression in all samples) nor a random (presence of mRNA from both alleles in all samples) pattern of XCI was observed in TF. Due to the importance of XCI in mammalian embryo development and its sensitivity to in vitro conditions, X-linked genes subject to XCI are candidates for use in the development of embryo quality molecular markers for assisted reproduction. (AU)

• Processo FAPESP: 10/07971-3 - Efeitos do cultivo in vitro e da nutrição sobre a reprogramação epigenética em ovócitos e embriões bovinos
• Beneficiário: Allice Rodrigues Ferreira
• Modalidade de apoio: Bolsas no Brasil - Doutorado