Low Level Laser Therapy Reduces the Development of Lung Inflammation Induced by Formaldehyde Exposure

Autor(es)

da Silva, Cristiane Miranda ^[1] ; Leal, Mayara Peres ^[1] ; Brochetti, Robson Alexandre ^[1] ; Braga, Tarcio ^[2] ; Vitoretti, Luana Beatriz ^[1] ; Saraiva Camara, Niels Olsen ^[2] ; Damazo, Amilcar Sabino ^[3] ; Ligeiro-de-Oliveira, Ana Paula ^[1] ; Chavantes, Maria Cristina ^[4] ; Lino-dos-Santos-Franco, Adriana ^[1]

Afiliação do(s) autor(es)

^[1] Univ Nove de Julho UNINOVE, Post Grad Program Biophoton Appl Hlth Sci, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Dept Immunol, Sao Paulo - Brazil

^[3] Fed Univ Cuiaba, Dept Basic Sci Hlth, Fac Med Sci, Cuiaba - Brazil

^[4] Univ Nove de Julho UNINOVE, Post Grad Program Med, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 10, n. 11 NOV 16 2015.
• Citações Web of Science: 19

Resumo

Lung diseases constitute an important public health problem and its growing level of concern has led to efforts for the development of new therapies, particularly for the control of lung inflammation. Low Level Laser Therapy (LLLT) has been highlighted as a non-invasive therapy with few side effects, but its mechanisms need to be better understood and explored. Considering that pollution causes several harmful effects on human health, including lung inflammation, in this study, we have used formaldehyde (FA), an environmental and occupational pollutant, for the induction of neutrophilic lung inflammation. Our objective was to investigate the local and systemic effects of LLLT after FA exposure. Male Wistar rats were exposed to FA (1%) or vehicle (distillated water) during 3 consecutive days and treated or not with LLLT (1 and 5 hours after each FA exposure). Non-manipulated rats were used as control. 24 h after the last FA exposure, we analyzed the local and systemic effects of LLLT. The treatment with LLLT reduced the development of neutrophilic lung inflammation induced by FA, as observed by the reduced number of leukocytes, mast cells degranulated, and a decreased myeloperoxidase activity in the lung. Moreover, LLLT also reduced the microvascular lung permeability in the parenchyma and the intrapulmonary bronchi. Alterations on the profile of inflammatory cytokines were evidenced by the reduced levels of IL-6 and TNF-alpha and the elevated levels of IL-10 in the lung. Together, our results showed that LLLT abolishes FA-induced neutrophilic lung inflammation by a reduction of the inflammatory cytokines and mast cell degranulation. This study may provide important information about the mechanisms of LLLT in lung inflammation induced by a pollutant. (AU)

• Processo FAPESP: 15/00830-9 - Efeitos da exposição à poluição ambiental/ocupacional sobre a fibrose pulmonar idiopática
• Beneficiário: Adriana Lino dos Santos Franco
• Linha de fomento: Auxílio à Pesquisa - Regular
• Processo FAPESP: 12/16498-5 - Efeito da terapia com laser de baixa potência (LBP) em modelos experimentais de doenças pulmonares crônicas
• Beneficiário: Ana Paula Ligeiro de Oliveira
• Linha de fomento: Auxílio à Pesquisa - Apoio a Jovens Pesquisadores
• Processo FAPESP: 11/51711-9 - Exposição in utero à poluição ambiental e ocupacional e sua repercussão para o desencadeamento de inflamação alérgica pulmonar na prole: correlação com mecanismos epigenéticos
• Beneficiário: Adriana Lino dos Santos Franco
• Linha de fomento: Auxílio à Pesquisa - Apoio a Jovens Pesquisadores