Neutrophil-derived microvesicles enter cartilage and protect the joint in inflammatory arthritis

Autor(es):Mostrar menos -

Headland, Sarah E. ^[1] ; Jones, Hefin R. ^[1] ; Norling, Lucy V. ^[1] ; Kim, Andrew ^[2] ; Souza, Patricia R. ^[1] ; Corsiero, Elisa ^[1] ; Gil, Cristiane D. ^[3] ; Nerviani, Alessandra ^[1] ; Dell'Accio, Francesco ^{[1, 4]} ; Pitzalis, Costantino ^{[1, 4]} ; Oliani, Sonia M. ^[3] ; Jan, Lily Y. ^[2] ; Perretti, Mauro ^[1]

Afiliação do(s) autor(es)

^[1] Queen Mary Univ London, William Harvey Res Inst, Barts & London Sch Med, London EC1M 6BQ - England

^[2] Univ Calif San Francisco, Howard Hughes Med Inst, Dept Physiol, San Francisco, CA 94143 - USA

^[3] Sao Paulo State Univ UNESP, Dept Biol, Inst Biociencias Letras & Ciencias Exatas, BR-15054000 Sao Jose Do Rio Preto - Brazil

^[4] Barts Hlth Trust, Dept Rheumatol, London E1 4DG - England

• Tipo de documento: Artigo Científico
• Fonte: Science Translational Medicine; v. 7, n. 315 NOV 25 2015.
• Citações Web of Science: 77

Resumo

Microvesicles (MVs) are emerging as a new mechanismof intercellular communication by transferring cellular lipid and protein components to target cells, yet their function in disease is only now being explored. We found that neutrophilderived MVs were increased in concentration in synovial fluid from rheumatoid arthritis patients compared to paired plasma. Synovial MVs overexpressed the proresolving, anti-inflammatory protein annexin A1 (AnxA1). Mice deficient in TMEM16F, a lipid scramblase required for microvesiculation, exhibited exacerbated cartilage damage when subjected to inflammatory arthritis. To determine the function of MVs in inflammatory arthritis, toward the possibility of MV-based therapeutics, we examined the role of immune cell-derived MVs in rodent models and in human primary chondrocytes. In vitro, exogenous neutrophil-derived AnxA1(+) MVs activated anabolic gene expression in chondrocytes, leading to extracellular matrix accumulation and cartilage protection through the reduction in stress-adaptive homeostatic mediators interleukin-8 and prostaglandin E-2. In vivo, intra-articular injection of AnxA1(+) MV lessened cartilage degradation caused by inflammatory arthritis. Arthritic mice receiving adoptive transfer of whole neutrophils displayed abundant MVs within cartilage matrix and revealed that MVs, but not neutrophils themselves, can penetrate cartilage. Mechanistic studies support a model whereby MV-associated AnxA1 interacts with its receptor FPR2 (formyl peptide receptor 2)/ALX, increasing transforming growth factor-beta production by chondrocytes, ultimately leading to cartilage protection. We envisage that MVs, either directly or loaded with therapeutics, can be harnessed as a unique therapeutic strategy for protection in diseases associated with cartilage degeneration. (AU)

• Processo FAPESP: 12/21603-2 - Bioengenharia tecidual: avaliar os transplantes heterólogos de pele usando matrizes acelulares suínas e a proteína anexina A1 como potencial recurso terapêutico nos processos regenerativo e cicatricial
• Beneficiário: Sonia Maria Oliani
• Modalidade de apoio: Auxílio à Pesquisa - Regular