Distribution of glutamate receptors in the posterodorsal medial amygdala of adult male rats

Autor(es)

Dalpian, Francine ^[1] ; Brusco, Janaina ^[2] ; Calcagnotto, Maria Elisa ^[3] ; Moreira, Jorge E. ^[2] ; Rasia-Filho, Alberto A. ^[1]

Afiliação do(s) autor(es)

^[1] Fed Univ Hlth Sci Porto Alegre UFCSPA, Dept Basic Sci Physiol, Porto Alegre, RS - Brazil

^[2] Univ Sao Paulo MRP USP, Ribeirao Preto Sch Med, Deparments Pathol & Forens Med & Neurosci & Behav, Lab Synapt Struct, BR-14049900 Sao Paulo, SP - Brazil

^[3] Univ Fed Rio Grande do Sul, Dept Biochem, Porto Alegre, RS - Brazil

• Tipo de documento: Artigo Científico
• Fonte: HISTOLOGY AND HISTOPATHOLOGY; v. 30, n. 11, p. 1303-1311, NOV 2015.
• Citações Web of Science: 6

Resumo

The rat posterodorsal medial amygdala (MePD) has a remarkable neuronal plasticity and responds to olfactory/pheromonal stimuli to modulate emotional and reproductive behaviors. Glutamate is locally released by incoming sensorial pathways to establish and enforce synaptic inputs. Here, we combined Dil dye and immunolabeling procedure under confocal microscopy to describe the presence and distribution of glutamate receptors on neurons of the MePD of adult male rats. Western blot analysis interrogated binding specificity. Both AMPA (GluA1-4 subunits) and NMDA (GluN1 subunit) receptors were immunolabeled on cell bodies and along proximal and distal dendritic shafts. AMPA receptors were mainly observed on mushroom and stubby/wide spines, whereas NMDA receptors were found on thin spines. Colocalization of AMPA and NMDA receptors occurred in some spines. Filopodium did not show immunolabeled puncta on it. Our results are different from the distribution of glutamate receptors in the amygdaloid lateral nucleus, an upstream area involved with emotional processing, and suggest a region-specific excitatory transmission at proximal and distal dendritic branches. Altogether, these data provide new information for synaptic processing in the MePD likely related to the modulation of social behavior in rats. (AU)

• Processo FAPESP: 05/56447-7 - Pesquisa através de imagens por ressonância magnética de alto campo destinadas a estudos em humanos
• Beneficiário: João Pereira Leite
• Modalidade de apoio: Auxílio à Pesquisa - Programa CINAPCE - Temático
• Processo FAPESP: 09/01571-6 - Amígdala medial de ratas ao longo do ciclo estral: morfologia, expressão de mRNAs, proteínas sinápticas e lateralidade
• Beneficiário: Jorge Eduardo Moreira
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 12/19011-0 - Estudo dos espinhos dendríticos na amígdala medial póstero-dorsal de ratos: morfologia e conectividade
• Beneficiário: Jorge Eduardo Moreira
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/10753-0 - Neurogênese e plasticidade sináptica no hipocampo de ratos submetidos à separação maternal e ao ambiente enriquecido
• Beneficiário: Jorge Eduardo Moreira
• Modalidade de apoio: Auxílio à Pesquisa - Regular