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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Distribution of glutamate receptors in the posterodorsal medial amygdala of adult male rats

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Author(s):
Dalpian, Francine [1] ; Brusco, Janaina [2] ; Calcagnotto, Maria Elisa [3] ; Moreira, Jorge E. [2] ; Rasia-Filho, Alberto A. [1]
Total Authors: 5
Affiliation:
[1] Fed Univ Hlth Sci Porto Alegre UFCSPA, Dept Basic Sci Physiol, Porto Alegre, RS - Brazil
[2] Univ Sao Paulo MRP USP, Ribeirao Preto Sch Med, Deparments Pathol & Forens Med & Neurosci & Behav, Lab Synapt Struct, BR-14049900 Sao Paulo, SP - Brazil
[3] Univ Fed Rio Grande do Sul, Dept Biochem, Porto Alegre, RS - Brazil
Total Affiliations: 3
Document type: Journal article
Source: HISTOLOGY AND HISTOPATHOLOGY; v. 30, n. 11, p. 1303-1311, NOV 2015.
Web of Science Citations: 6
Abstract

The rat posterodorsal medial amygdala (MePD) has a remarkable neuronal plasticity and responds to olfactory/pheromonal stimuli to modulate emotional and reproductive behaviors. Glutamate is locally released by incoming sensorial pathways to establish and enforce synaptic inputs. Here, we combined Dil dye and immunolabeling procedure under confocal microscopy to describe the presence and distribution of glutamate receptors on neurons of the MePD of adult male rats. Western blot analysis interrogated binding specificity. Both AMPA (GluA1-4 subunits) and NMDA (GluN1 subunit) receptors were immunolabeled on cell bodies and along proximal and distal dendritic shafts. AMPA receptors were mainly observed on mushroom and stubby/wide spines, whereas NMDA receptors were found on thin spines. Colocalization of AMPA and NMDA receptors occurred in some spines. Filopodium did not show immunolabeled puncta on it. Our results are different from the distribution of glutamate receptors in the amygdaloid lateral nucleus, an upstream area involved with emotional processing, and suggest a region-specific excitatory transmission at proximal and distal dendritic branches. Altogether, these data provide new information for synaptic processing in the MePD likely related to the modulation of social behavior in rats. (AU)

FAPESP's process: 05/56447-7 - Research through images from high field magnetic resonance aimed at studies in humans
Grantee:João Pereira Leite
Support Opportunities: Inter-institutional Cooperation in Support of Brain Research (CINAPCE) - Thematic Grants
FAPESP's process: 09/01571-6 - The postero-dorsal medial amigdala of rats during the estrus cycle: morfology, mRNA expression, synaptic proteins and laterality
Grantee:Jorge Eduardo Moreira
Support Opportunities: Regular Research Grants
FAPESP's process: 12/19011-0 - Dendritic spines in the posterodorsal medial amygdala of rats: morphology and connectivity
Grantee:Jorge Eduardo Moreira
Support Opportunities: Regular Research Grants
FAPESP's process: 11/10753-0 - Neurogenesis and synaptic plasticity in the hippocampus of rats submitted to the maternal separation and enriched environment
Grantee:Jorge Eduardo Moreira
Support Opportunities: Regular Research Grants