Changes in T-cell phenotype and cytokines profile in maternal blood, cord blood and colostrum of diabetic mothers

Autor(es)

Gomes Fagundes, Danny Laura ^[1] ; Franca, Eduardo Luzia ^[2] ; da Silva Fernandes, Rubian Trindade ^[2] ; Pernet Hara, Cristiane de Castro ^[2] ; Morceli, Glilciane ^[1] ; Honorio-Franca, Adenilda Cristina ^[2] ; Paranhos Calderon, Iracema de Mattos ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Botucatu Med Sch, Grad Program Gynecol Obstet & Mastol, Sao Paulo - Brazil

^[2] Univ Fed Mato Grosso, Inst Biol & Hlth Sci, Barra Do Garcas, Mato Grosso - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Maternal-Fetal & Neonatal Medicine; v. 29, n. 6, p. 998-1004, MAR 18 2016.
• Citações Web of Science: 8

Resumo

Objective: The present study evaluated the cells and cytokine of maternal blood, cord blood and colostrum of diabetic mothers.Methods: The women evaluated were divided according to their body mass index (BMI) and glycemic status into non-diabetic (ND - N=15), mild gestational hyperglycemic (MGH - N=15), diabetes mellitus gestational (DMG - N=13) and type-2 diabetes mellitus (DM2 - N=15) groups. The subsets of cells and cytokine profile were determined by flow cytometry.Results: Maternal blood from MGH group had increase percentage of CD3(+)T cells, and DM-2 group had decrease percentage of CD4(+) T cells. The cord blood from hyperglycemic groups showed lower percentage of CD3(+) T cells expressing CD45RO(+) and higher of CD4(+) T cells and CD4(+) T cells expressing CD45RA(+). In the colostrum, the CD4(+) T cells and CD4(+) T cells expressed CD45RA(+) increase in hyperglycemic groups. The DM2 group exhibited higher IL17 levels in maternal blood. IFN- was lower in cord blood from MGH and DMG groups with overweight/obese. Irrespective of the glycemic status, IL6 was higher in colostrum.Conclusion: The results obtained suggest that maternal hyperglycemia modifies the phenotypes of T cells and cytokines profile in maternal, cord blood and colostrum. (AU)

• Processo FAPESP: 12/24212-4 - Marcadores da imunomodulação no sangue materno e fetal e nas placentas de mães diabéticas ou com hiperglicemia gestacional leve
• Beneficiário: Cristiane de Castro Pernet Hara
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 12/18033-0 - Marcadores da imunomodulação no sangue materno e fetal e nas placentas de mães diabéticas ou com hiperglicemia gestacional leve
• Beneficiário: Glilciane Morceli
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado