Potential of the Lectin/Inhibitor Isolated from Crataeva tapia Bark (CrataBL) for Controlling Callosobruchus maculatus Larva Development

Autor(es)

Nunes, Natalia N. S. ^[1] ; Ferreira, Rodrigo S. ^[1] ; Silva-Lucca, Rosemeire A. ^[2] ; de Sa, Leonardo F. R. ^[3] ; de Oliveira, Antonia Elenir A. ^[3] ; Correia, Maria Tereza dos S. ^[4] ; Paiva, Patricia Maria G. ^[4] ; Wlodawer, Alexander ^[5] ; Oliva, Maria Luiza V. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, UNIFESP, Dept Bioquim, EPM, BR-04044020 Sao Paulo, SP - Brazil

^[2] Univ Estadual Oeste do Parana, Ctr Engn & Ciencias Exatas, Toledo, Parana - Brazil

^[3] Univ Estadual Norte Fluminense Darcy Ribeiro UENF, CBB, Lab Quim & Funcao Prot & Peptideos, Campos Dos Goytacazes, RJ - Brazil

^[4] Univ Fed Pernambuco, Dept Bioquim, Recife, PE - Brazil

^[5] NCI, Macromol Crystallog Lab, Ctr Canc Res, Frederick, MD 21702 - USA

• Tipo de documento: Artigo Científico
• Fonte: Journal of Agricultural and Food Chemistry; v. 63, n. 48, p. 10431-10436, DEC 9 2015.
• Citações Web of Science: 6

Resumo

Callosobruchus maculatus is an important predator of cowpeas. Due to infestation during storage, this insect affects the quality of seed and crop yield. This study aimed to investigate the effects of CrataBL, a multifunction protein isolated from Crataeva tapia bark, on C. maculatus larva development. The protein, which is stable even in extreme pH conditions, showed toxic activity, reducing the larval mass 45 and 70% at concentrations of 0.25 and 1.0% (w/w), respectively. Acting as an inhibitor, CrataBL decreased by 39% the activity of cysteine proteinases from larval gut. Conversely, the activity of serine proteinases was increased about 8-fold. The toxic properties of CrataBL may also be attributed to its capacity of binding to glycoproteins or glycosaminoglycans. Such binding interferes with larval metabolism, because CrataBL FITC was found in the fat body, Malpighian tubules, and feces of larvae. These results demonstrate the potential of this protein for controlling larva development. (AU)

• Processo FAPESP: 09/17058-6 - Purificação e caracterização de compostos isolados de plantas com atividade larvicida.
• Beneficiário: Natalia Neto dos Santos Nunes
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 09/53766-5 - Proteínas de origem vegetal com seletividade para inibição de enzimas de mamíferos e seu papel como agente anti-inflamatório, antitrombótico, antidiabético e antitumoral
• Beneficiário: Maria Luiza Vilela Oliva
• Modalidade de apoio: Auxílio à Pesquisa - Temático