Randomized in vivo evaluation of photodynamic antimicrobial chemotherapy on deciduous carious dentin

Autor(es)

Steiner-Oliveira, Carolina ^[1] ; Longo, Priscila Larcher ^[2] ; Correa Aranha, Ana Cecilia ^[3] ; Ramalho, Karen Mueller ^[3] ; Alves Mayer, Marcia Pinto ^[2] ; Eduardo, Carlos de Paula ^[3]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Pediat Dent, BR-13414903 Piracicaba, SP - Brazil

^[2] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, BR-05508000 Sao Paulo, SP - Brazil

^[3] Univ Sao Paulo, Sch Dent, Special Lab Lasers Dent, Restorat Dept, BR-05508000 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Biomedical Optics; v. 20, n. 10 OCT 2015.
• Citações Web of Science: 13

Resumo

The aim of this randomized in vivo study was to compare antimicrobial chemotherapies in primary carious dentin. Thirty-two participants ages 5 to 7 years underwent partial caries removal from deep carious dentin lesions in primary molars and were subsequently divided into three groups: control {[}chlorhexidine and resin-modified glass ionomer cement (RMGIC)], LEDTB {[}photodynamic antimicrobial chemotherapy (PACT) with light-emitting diode associated with toluidine blue solution and RMGIC], and LMB {[}PACT with laser associated with methylene blue solution and RMGIC]. The participants were submitted to initial clinical and radiographic examinations. Demographic features and biofilm, gingival, and DMFT/DMFS indexes were evaluated, in addition to clinical and radiographic followups at 6 and 12 months after treatments. Carious dentin was collected before and after each treatment, and the number of Streptococcus mutans, Streptococcus sobrinus, Lactobacillus casei, Fusobacterium nucleatum, Atopoblum rimae, and total bacteria was established by quantitative polymerase chain reaction. No signs of pain or restoration failure were observed. All therapies were effective in reducing the number of microorganisms, except for S. sobrinus. No statistical differences were observed among the protocols used. All therapies may be considered as effective modern approaches to minimal intervention for the management of deep primary caries treatment. (C) 2015 Society of Photo-Optical Instrumentation Engineers (SPIE) (AU)

• Processo FAPESP: 11/08392-0 - Estudo randomizado do efeito antimicrobiano in vivo da terapia fotodinâmica na dentina cariada de dentes decíduos: análise microbiológica, clínica e radiográfica
• Beneficiário: Carlos de Paula Eduardo
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 10/07212-5 - Estudo randomizado do efeito antimicrobiano in vivo da terapia fotodinâmica na dentina cariada de dentes decíduos. Análise microbiológica, clínica e radiográfica.
• Beneficiário: Carolina Steiner Oliveira Alarcon
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado