Copaifera langsdorffii oleoresin and its isolated compounds: antibacterial effect and antiproliferative activity in cancer cell lines

Autor(es)

Abrao, Fariza ^[1] ; de Araujo Costa, Luciana Delfino ^[1] ; Alves, Jacqueline Morais ^[2] ; Senedese, Juliana Marques ^[2] ; de Castro, Pamela Tinti ^[2] ; Ambrosio, Sergio Ricardo ^[3] ; Sola Veneziani, Rodrigo Cassio ^[3] ; Bastos, Jairo Kenupp ^[4] ; Tavares, Denise Crispim ^[2] ; Martins, Carlos Henrique G. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Franca, Lab Pesquisa Microbiol Aplicada, Franca, SP - Brazil

^[2] Univ Franca, Lab Mutagenese, Franca, SP - Brazil

^[3] Univ Franca, Grp Pesquisa Prod Nat, Franca, SP - Brazil

^[4] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, BR-14049 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE; v. 15, DEC 21 2015.
• Citações Web of Science: 11

Resumo

Background: Natural products display numerous therapeutic properties (e.g., antibacterial activity), providing the population with countless benefits. Therefore, the search for novel biologically active, naturally occurring compounds is extremely important. The present paper describes the antibacterial action of the Copaifera langsdorffii oleoresin and ten compounds isolated from this oleoresin against multiresistant bacteria; it also reports the antiproliferative activity of the Copaifera langsdorffii oleoresin and (-)-copalic acid. Methods: MICs and MBCs were used to determine the antibacterial activity. Time-kill curve assays provided the time that was necessary for the bacteria to die. The Minimum Inhbitory Concentration of Biofilm (CIMB50) of the compounds that displayed the best results was calculated. Cytotoxicity was measured by using the XTT assay. Results: The diterpene (-)-copalic acid was the most active antibacterial and afforded promising Minimum Inhibitory Concentration (MIC) values for most of the tested strains. Determination of the bactericidal kinetics against some bacteria revealed that the bactericidal effect emerged within six hours of incubation for Streptococcus pneumoniae. Concerning the antibiofilm action of this diterpene, its MICB50 was twofold larger than its CBM against S. capitis and S. pneumoniae. The XTT assay helped to evaluate the cytotoxic effect; results are expressed as IC50. The most pronounced antiproliferative effect arose in tumor cell lines treated with (-)-copalic acid; the lowest IC50 value was found for the human glioblastoma cell line. Conclusions: The diterpene (-)-copalic acid is a potential lead for the development of new selective antimicrobial agents to treat infections caused by Gram-positive multiresistant microorganisms, in both the sessile and planktonic mode. This diterpene is also a good candidate to develop anticancer drugs. (AU)

• Processo FAPESP: 11/13630-7 - Validação química e farmacológica de extratos e princípios ativos de espécies de Copaifera
• Beneficiário: Jairo Kenupp Bastos
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 11/09229-5 - Efeito antibacteriano in vitro do óleo-resina de copaíba (Copaifera langsdorfii) e princípios ativos frente a bactérias multirresistentes
• Beneficiário: Fariza Abrão
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 09/17237-8 - Avaliação in vivo e in vitro do potencial citotóxico, mutagênico, genotóxico e do efeito quimiopreventivo do óleo da Copaifera lansgdorffii e do diterpeno ácido copálico
• Beneficiário: Jacqueline Morais Alves
• Modalidade de apoio: Bolsas no Brasil - Doutorado