Microsatellite loci cross-species transferability in Aedes fluviatilis (Diptera:Culicidae): a cost-effective approach for population genetics studies

Autor(es)

Multini, Laura Cristina ^[1] ; Marrelli, Mauro Toledo ^{[2, 1]} ; Bruno Wilke, Andre Barretto ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Trop Med Sao Paulo, BR-05403000 Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Sch Publ Hlth, Dept Epidemiol, BR-01246904 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PARASITES & VECTORS; v. 8, DEC 15 2015.
• Citações Web of Science: 5

Resumo

Background: Aedes fluviatilis is a neotropical mosquito species thought to be a potential vector of Yellow Fever viruses and can be infected with Plasmodium gallinaceum in laboratory. A better understanding of its genetic structure is very important to understand its epidemiologic potential and how it is responding to urbanization. The objective of this study was to survey the transferability of microsatellites loci developed for other Aedes to Ae. fluviatilis. Findings: We tested in Ae. fluviatilis 40 pairs of primers known to flank microsatellite regions in Aedes aegypti, Aedes albopictus and Aedes caspius, and found eight loci that amplified consistently. The number of alleles per locus ranged from 2 to 15, and the expected heterozygosity ranged from 0.09 to 0.85. Conclusions: We found that several microsatellite primers successfully transferred to Ae. fluviatilis. This finding opens avenues for cost-effective optimization of high-resolution population genetic tools. (AU)

• Processo FAPESP: 12/19117-2 - Paratransgênese em mosquitos Culex quinquefasciatus
• Beneficiário: André Barretto Bruno Wilke
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 13/15313-4 - Análise da estrutura populacional de mosquitos (Diptera: Culicidae) utilizando marcadores microssatélites e morfometria alar
• Beneficiário: Mauro Toledo Marrelli
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/22347-2 - Caracterização genética de populações de Aedes fluviatilis de parques municipais em São Paulo, utilizando marcadores microssatélites
• Beneficiário: Laura Cristina Multini
• Modalidade de apoio: Bolsas no Brasil - Mestrado