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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Microsatellite loci cross-species transferability in Aedes fluviatilis (Diptera:Culicidae): a cost-effective approach for population genetics studies

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Author(s):
Multini, Laura Cristina [1] ; Marrelli, Mauro Toledo [2, 1] ; Bruno Wilke, Andre Barretto [2]
Total Authors: 3
Affiliation:
[1] Univ Sao Paulo, Inst Trop Med Sao Paulo, BR-05403000 Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Sch Publ Hlth, Dept Epidemiol, BR-01246904 Sao Paulo, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: PARASITES & VECTORS; v. 8, DEC 15 2015.
Web of Science Citations: 5
Abstract

Background: Aedes fluviatilis is a neotropical mosquito species thought to be a potential vector of Yellow Fever viruses and can be infected with Plasmodium gallinaceum in laboratory. A better understanding of its genetic structure is very important to understand its epidemiologic potential and how it is responding to urbanization. The objective of this study was to survey the transferability of microsatellites loci developed for other Aedes to Ae. fluviatilis. Findings: We tested in Ae. fluviatilis 40 pairs of primers known to flank microsatellite regions in Aedes aegypti, Aedes albopictus and Aedes caspius, and found eight loci that amplified consistently. The number of alleles per locus ranged from 2 to 15, and the expected heterozygosity ranged from 0.09 to 0.85. Conclusions: We found that several microsatellite primers successfully transferred to Ae. fluviatilis. This finding opens avenues for cost-effective optimization of high-resolution population genetic tools. (AU)

FAPESP's process: 12/19117-2 - Paratransgenesis in Culex quinquefasciatus mosquitoes
Grantee:André Barretto Bruno Wilke
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 13/15313-4 - Analysis of the population structure of mosquitoes (Diptera: Culicidae) using microsatellite markers and wing morphometry
Grantee:Mauro Toledo Marrelli
Support Opportunities: Regular Research Grants
FAPESP's process: 13/22347-2 - Genetic characterisation of Aedes fluviatilis populations from municipal parks in São Paulo, using microsatellite markers
Grantee:Laura Cristina Multini
Support Opportunities: Scholarships in Brazil - Master