Analysis of the population structure of mosquitoes (Diptera: Culicidae) using microsatellite markers and wing morphometry

• Grant number: 13/15313-4
• Support Opportunities: Regular Research Grants
• Duration: October 01, 2013 - March 31, 2016
• Field of knowledge: Biological Sciences - Parasitology - Entomology and Malacology of Parasites and Vectors
• Principal Investigator: Mauro Toledo Marrelli
• Grantee: Mauro Toledo Marrelli
• Host Institution: Faculdade de Saúde Pública (FSP). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated researchers: Lincoln Suesdek Rocha

Abstract

Vectors of agent of diseases associated with humans expanded their territory, leading to the introduction of mosquito species causing epidemics in new areas. A better understanding of the genetic structure of mosquito populations can help anticipate the distribution of mosquito-borne diseases and have key role in epidemiological interventions. Microsatellite markers and morphometric analysis of wings can be used to analyze the genetic structure of populations of mosquitoes. Previous results of our group using these markers indicate that there must be significant differences between populations of mosquitoes collected in the ten parks in the present study, since present distinct ecosystems. After the monthly collections made between March 2011 and February 2012, in seven parks (Anhanguera, Alfredo Volpi, Carmo, Chico Mendes, Ibirapuera, Santo Dias and Shangrilá), and between August 2012 and July 2013 in three parks (Piqueri, Previdência and Burle Marx) during the a project on biodiversity of mosquitoes in municipal parks of São Paulo (FAPESP Project 2010/51230-8) it was verified that the species Culex quinquefasciatus, Cx. nigripalpus, Aedes fluviatilis, Ae. scapularis and Ae. albopictus were collected in all parks showing relatively high abundances. Whereas such areas showed varying degrees of diversity and, therefore, may differ in terms of epidemiological importance and due to the epidemiological importance and abundance of five species mentioned, this project was designed as a continuation of the biodiversity project, selecting the five species of mosquitoes for population analysis using microsatellite markers and morphometry of the wings. (AU)

Scientific publications

(13)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

OLIVEIRA-CHRISTE, RAFAEL; BRUNO WILKE, ANDRE BARRETTO; MARRELLI, MAURO TOLEDO. Microgeographic Wing-Shape Variation in Aedes albopictus and Aedes scapularis (Diptera: Culicidae) Populations. INSECTS, v. 11, n. 12, DEC 2020. (13/15313-4, 17/02342-7)

MULTINI, LAURA CRISTINA; DA SILVA DE SOUZA, ANA LETICIA; MARRELLI, MAURO TOLEDO; BRUNO WILKE, ANDRE BARRETTO. Population structuring of the invasive mosquito Aedes albopictus (Diptera: Culicidae) on a microgeographic scale. PLoS One, v. 14, n. 8, AUG 2 2019. (13/15313-4, 15/23386-7)

WILKE, ANDRE BARRETTO BRUNO; DE CARVALHO, GABRIELA CRISTINA; MARRELLI, MAURO TOLEDO. Retention of ancestral polymorphism in Culex nigripalpus (Diptera: Culicidae) from Sao Paulo, Brazil. INFECTION GENETICS AND EVOLUTION, v. 65, p. 333-339, NOV 2018. (13/15313-4, 13/18965-2)

WILK-DA-SILVA, RAMON; DE SOUZA LEAL DINIZ, MORGANA MICHELE CAVALCANTI; MARRELLI, MAURO TOLEDO; BRUNO WILKE, ANDRE BARRETTO. Wing morphometric variability in Aedes aegypti (Diptera: Culicidae) from different urban built environments. PARASITES & VECTORS, v. 11, OCT 26 2018. (15/01172-5, 13/15313-4)

BRUNO WILKE, ANDRE BARRETTO; CHRISTE, RAFAEL DE OLIVEIRA; MULTINI, LAURA CRISTINA; VIDAL, PALOMA OLIVEIRA; WILK-DA-SILVA, RAMON; DE CARVALHO, GABRIELA CRISTINA; MARRELLI, MAURO TOLEDO. Morphometric Wing Characters as a Tool for Mosquito Identification. PLoS One, v. 11, n. 8, AUG 23 2016. (13/15313-4, 12/19117-2)

MULTINI, LAURA CRISTINA; BRUNO WILKE, ANDRE BARRETTO; SUESDEK, LINCOLN; MARRELLI, MAURO TOLEDO. Population Genetic Structure of Aedes fluviatilis (Diptera: Culicidae). PLoS One, v. 11, n. 9, SEP 6 2016. (13/15313-4, 12/19117-2, 13/22347-2)

BRUNO WILKE, ANDRE BARRETTO; DE CARVALHO, GABRIELA CRISTINA; MARRELLI, MAURO TOLEDO. Microgeographic Population Structuring of Culex quinquefasciatus (Diptera: Culicidae) From Sao Paulo, Brazil. Journal of Medical Entomology, v. 54, n. 6, p. 1582-1588, NOV 2017. (13/18965-2, 13/15313-4)

MULTINI, LAURA CRISTINA; MARRELLI, MAURO TOLEDO; BRUNO WILKE, ANDRE BARRETTO. Microsatellite loci cross-species transferability in Aedes fluviatilis (Diptera:Culicidae): a cost-effective approach for population genetics studies. PARASITES & VECTORS, v. 8, DEC 15 2015. (12/19117-2, 13/15313-4, 13/22347-2)

CHRISTE, RAFAEL DE OLIVEIRA; BRUNO WILKE, ANDRE BARRETTO; VIDAL, PALOMA OLIVEIRA; MARRELLI, MAURO TOLEDO. Wing sexual dimorphism in Aedes fluviatilis (Diptera: Culicidae). INFECTION GENETICS AND EVOLUTION, v. 45, p. 434-436, NOV 2016. (12/19117-2, 13/15313-4)

CHRISTE, RAFAEL DE OLIVEIRA; MARRELLI, MAURO TOLEDO; VIDAL, PALOMA OLIVEIRA; VENDRAMI, DANIEL PAGOTTO; WILKE, ANDRE BARRETTO BRUNO. Assessment of the microgeographic population structure of Aedes fluviatilis (Diptera: Culicidae) using wing geometric morphometrics. ENTOMOLOGIA GENERALIS, v. 39, n. 3-4, p. 183-191, 2019. (13/15313-4)

BRUNO WILKE, ANDRE BARRETTO; WILK-DA-SILVA, RAMON; MARRELLI, MAURO TOLEDO. Microgeographic population structuring of Aedes aegypti (Diptera: Culicidae). PLoS One, v. 12, n. 9, SEP 20 2017. (12/19117-2, 13/15313-4, 15/01172-5)

DE CARVALHO, GABRIELA CRISTINA; VENDRAMI, DANIEL PAGOTTO; MARRELLI, MAURO TOLEDO; BRUNO WILKE, ANDRE BARRETTO. Wing variation in Culex nigripalpus (Diptera: Culicidae) in urban parks. PARASITES & VECTORS, v. 10, SEP 18 2017. (13/18965-2, 13/15313-4)

DE CARVALHO, GABRIELA CRISTINA; MARRELLI, MAURO TOLEDO; BRUNO WILKE, ANDRE BARRETTO. Microsatellite Markers Transferability in Culex nigripalpus (Diptera: Culicidae). Journal of Medical Entomology, v. 55, n. 5, p. 1334-1336, SEP 2018. (13/18965-2, 13/15313-4)