In vitro protective effect of lactic acid bacteria on Listeria monocytogenes adhesion and invasion of Caco-2 cells

Autor(es)

Winkelstroeter, L. K. ^[1] ; De Martinis, E. C. P. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, FCFRP, BR-14040903 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BENEFICIAL MICROBES; v. 6, n. 4, p. 535-542, 2015.
• Citações Web of Science: 3

Resumo

The adhesion of Listeria monocytogenes to intestinal endothelial cells is a crucial step in the infection process, which is not well understood. In this study, we evaluated the potential ability of bacteriocin-producing Enterococcus faecium, Leuconostoc mesenteroides and Lactobacillus sakei strains to prevent the adhesion and invasion of eukaryotic cells by ten different L. monocytogenes isolates. The results showed that E. faecium 130 co-cultured with L. monocytogenes was the most effective in preventing infection of Caco-2 cells, as the vast majority of isolates showed significantly lower adhesion counts and invasion rates below the quantification limit of the method (<30 cfu/plate). L. sakei 1 was the least effective strain in preventing L. monocytogenes infection; only one isolate presented a lower adhesion rate and two isolates reduced the invasion rate of Caco-2 cells. Fluorescence in situ hybridisation (FISH) assay was shown to be an effective tool to illustrate and identify species in co-culture with L. monocytogenes during the adhesion process to Caco-2 cells. (AU)

• Processo FAPESP: 08/58300-1 - Analise de expressao genica, formacao de biofilmes e adesao/invasao a celulas caco-2 por listeria monocytogenes em diferentes condicoes encontradas no trato gastrintestinal,em alimentos e em presenca...
• Beneficiário: Lizziane Kretli Winkelströter Eller
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 09/51141-8 - Análise de expressão gênica, formação de biofilme e adesão a células Caco-2 por Listeria monocytogenes em diferentes condições encontradas em alimentos
• Beneficiário: Elaine Cristina Pereira de Martinis
• Modalidade de apoio: Auxílio à Pesquisa - Regular