Photodynamic inactivation of virulence factors of Candida strains isolated from patients with denture stomatitis

Autor(es)

Pereira, Cristiane Aparecida ^[1] ; Domingues, Nadia ^[1] ; Silva, Michelle Peneluppi ^[1] ; Borges Pereira Costa, Anna Carolina ^[1] ; Junqueira, Juliana Campos ^[1] ; Cardoso Jorge, Antonio Olavo ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, Inst Sci & Technol, Sch Dent, Dept Biosci & Oral Diag, UNESP, BR-12245000 Sao Jose Dos Campos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY; v. 153, p. 82-89, DEC 2015.
• Citações Web of Science: 4

Resumo

Candida species are major microorganisms isolated in denture stomatitis (DS), an inflammatory process of the mucosa underlying removable dental prostheses, and express a variety of virulence factors that can increase their pathogenicity. The potential of Photodynamic inactivation (PDI) in planktonic culture, biofilms and virulence factors of Candida strains was evaluated. A total of 48 clinical Candida isolates from individuals wearing removable maxillary prostheses with DS were included in the study. The effects of erythrosine (ER, 200 mu M) and a green LED (lambda 532 +/- 10 nm, 237 mW/cm(2) and 42.63 J/cm(2)) in a planktonic culture were evaluated. The effect of the addition of ER at a concentration of 400 mu M together with a green LED was evaluated in biofilms. The virulence factors of all of the Candida strains were evaluated before and after the PDI process in cells derived from biofilm and planktonic assays. All of the Candida species were susceptible to ER and green LED. However, the biofilm structures were more resistant to PDI than the planktonic cultures. PDI also promoted slight reductions in most of the virulence factors of C. albicans and some of the Candida tropicalis strains. These results suggest that the addition of PDI is effective for reducing yeasts and may also reduce the virulence of certain Candida species and decrease their pathogenicity. (C) 2015 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 10/00879-4 - MICRORGANISMOS OPORTUNISTAS EM LESÕES DE ESTOMATITE PROTÉTICA E INFLUÊNCIA DA TERAPIA FOTODINÂMICA NOS FATORES DE VIRULÊNCIA DE Candida spp.
• Beneficiário: Cristiane Aparecida Pereira Correia
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 11/21346-7 - GENES DE Candida albicans COM FUNÇÃO DESCONHECIDA QUANTO À FORMAÇÃO DE BIOFILME, CARACTERÍSTICAS BIOLÓGICAS E INTERAÇÃO PATÓGENO-HOSPEDEIRO
• Beneficiário: Anna Carolina Borges Pereira da Costa
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 13/07411-6 - Biofilmes polimicrobianos: estudo, caracterização, avaliação da patogenicidade em Galleria mellonella, e influência na expressão de genes de virulência de C. albicans.
• Beneficiário: Cristiane Aparecida Pereira Correia
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 10/08936-7 - MICRORGANISMOS OPORTUNISTAS EM LESÕES DE ESTOMATITE PROTÉTICA E INFLUÊNCIA DA TERAPIA FOTODINÂMICA NOS FATORES DE VIRULÊNCIA DE Candida spp.
• Beneficiário: Antonio Olavo Cardoso Jorge
• Modalidade de apoio: Auxílio à Pesquisa - Regular