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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Photodynamic inactivation of virulence factors of Candida strains isolated from patients with denture stomatitis

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Author(s):
Pereira, Cristiane Aparecida [1] ; Domingues, Nadia [1] ; Silva, Michelle Peneluppi [1] ; Borges Pereira Costa, Anna Carolina [1] ; Junqueira, Juliana Campos [1] ; Cardoso Jorge, Antonio Olavo [1]
Total Authors: 6
Affiliation:
[1] Univ Estadual Paulista, Inst Sci & Technol, Sch Dent, Dept Biosci & Oral Diag, UNESP, BR-12245000 Sao Jose Dos Campos, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY; v. 153, p. 82-89, DEC 2015.
Web of Science Citations: 4
Abstract

Candida species are major microorganisms isolated in denture stomatitis (DS), an inflammatory process of the mucosa underlying removable dental prostheses, and express a variety of virulence factors that can increase their pathogenicity. The potential of Photodynamic inactivation (PDI) in planktonic culture, biofilms and virulence factors of Candida strains was evaluated. A total of 48 clinical Candida isolates from individuals wearing removable maxillary prostheses with DS were included in the study. The effects of erythrosine (ER, 200 mu M) and a green LED (lambda 532 +/- 10 nm, 237 mW/cm(2) and 42.63 J/cm(2)) in a planktonic culture were evaluated. The effect of the addition of ER at a concentration of 400 mu M together with a green LED was evaluated in biofilms. The virulence factors of all of the Candida strains were evaluated before and after the PDI process in cells derived from biofilm and planktonic assays. All of the Candida species were susceptible to ER and green LED. However, the biofilm structures were more resistant to PDI than the planktonic cultures. PDI also promoted slight reductions in most of the virulence factors of C. albicans and some of the Candida tropicalis strains. These results suggest that the addition of PDI is effective for reducing yeasts and may also reduce the virulence of certain Candida species and decrease their pathogenicity. (C) 2015 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 10/00879-4 - OPPORTUNISTIC MICROORGANISMS IN INJURIES denture stomatitis AND INFLUENCE OF PHOTODYNAMIC THERAPY IN VIRULENCE FACTORS OF Candida spp.
Grantee:Cristiane Aparecida Pereira
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 11/21346-7 - GENES WITH UNKNOWN FUNCTION OF Candida albicans AS TO BIOFILM FORMATION, BIOLOGICAL CHARACTERISTICS AND HOST- PATHOGEN INTERACTION
Grantee:Anna Carolina Borges Pereira da Costa
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 13/07411-6 - Polymicrobial biofilms: study, characterization, evaluation of pathogenicity in Galleria mellonella, and influence in the expression of C. albicans virulence genes.
Grantee:Cristiane Aparecida Pereira
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 10/08936-7 - OPPORTUNISTIC MICROORGANISMS IN INJURIES denture stomatitis AND INFLUENCE OF PHOTODYNAMIC THERAPY IN VIRULENCE FACTORS OF Candida spp.
Grantee:Antonio Olavo Cardoso Jorge
Support type: Regular Research Grants