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Effects of the photodynamic therapy in the expression of the genes SAP5, PLB1 and LIP9 in Candida albicans

Grant number: 12/09188-0
Support type:Scholarships in Brazil - Master
Effective date (Start): August 01, 2012
Effective date (End): December 31, 2013
Field of knowledge:Biological Sciences - Microbiology - Applied Microbiology
Principal Investigator:Antonio Olavo Cardoso Jorge
Grantee:Fernanda Freire
Home Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil

Abstract

With the increasing number of strains of Candida ssp. resistant to antifungal agents, becomes important and necessary the accomplishment of researches that evaluate the effects of new therapeutic methods (PDT) about factors of virulence of that gender, as the production of the hydrolytic enzymes aspartyl-proteinase (SAP), lipase (LIP) and phospholipase (PL). Thus, the objective of this study will be to verify the effects of the photodynamic therapy on biofilms of Candida albicans, evaluating their effects on the expression of the genes SAP5, PLB1 and LIP9 for the yeast. Will be used clinical strains of Candida albicans (n=9) were isolated from patients bearers of the virus HIV and a pattern strain ATCC 18804, that are stored in the laboratory of Microbiology and Immunology, University of Dentistry of São José dos Campos/UNESP. The quantification of gene expression will be related to the production of hydrolytic enzymes (SAP5, PLB and LIP) in clinical samples and the reference strain using the RT-PCR assay in real time. The presence and expression of the genes will be analyzed before and after photodynamic therapy. For photodynamic therapy will be used laser-aluminum-gallium arsenide low power (red visible, 660 nm) as light source and the methylene blue to 300 µM, as a photosensitizer. They will be appraised four experimental groups, in triplicate, for each strain: a) F+L+: sensitization with methylene blue and laser irradiation; b) F+L-: only treatment with methylene blue; c) F-L+: only laser irradiation and d) F-L-: control group, without sensitization with methylene blue and light absence. The results will be analyzed statistically (p<0.05).

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
FREIRE, FERNANDA; DE BARROS, PATRICIA PIMENTEL; AVILA, DAMARA DA SILVA; BACK BRITO, GRAZIELLA NUERNBERG; JUNQUEIRA, JULIANA CAMPOS; CARDOSO JORGE, ANTONIO OLAVO. Evaluation of gene expression SAP5, LIP9, and PLB2 of Candida albicans biofilms after photodynamic inactivation. Lasers in Medical Science, v. 30, n. 5, p. 1511-1518, JUL 2015. Web of Science Citations: 6.
COSTA, ANNA C. B. P.; PEREIRA, CRISTIANE A.; FREIRE, FERNANDA; JUNQUEIRA, JULIANA C.; JORGE, ANTONIO O. C. Methods for obtaining reliable and reproducible results in studies of Candida biofilms formed in vitro. MYCOSES, v. 56, n. 6, p. 614-622, NOV 2013. Web of Science Citations: 22.
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
FREIRE, Fernanda. Efeitos da terapia fotodinâmica na expressão dos genes SAP5, LIP9 e PLB2 em Candida albicans. 2013. 54 f. Master's Dissertation - Universidade Estadual Paulista "Julio de Mesquita Filho". Instituto de Ciência e Tecnologia.

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