|Support type:||Scholarships in Brazil - Master|
|Effective date (Start):||August 01, 2012|
|Effective date (End):||December 31, 2013|
|Field of knowledge:||Biological Sciences - Microbiology - Applied Microbiology|
|Principal Investigator:||Antonio Olavo Cardoso Jorge|
|Home Institution:||Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil|
With the increasing number of strains of Candida ssp. resistant to antifungal agents, becomes important and necessary the accomplishment of researches that evaluate the effects of new therapeutic methods (PDT) about factors of virulence of that gender, as the production of the hydrolytic enzymes aspartyl-proteinase (SAP), lipase (LIP) and phospholipase (PL). Thus, the objective of this study will be to verify the effects of the photodynamic therapy on biofilms of Candida albicans, evaluating their effects on the expression of the genes SAP5, PLB1 and LIP9 for the yeast. Will be used clinical strains of Candida albicans (n=9) were isolated from patients bearers of the virus HIV and a pattern strain ATCC 18804, that are stored in the laboratory of Microbiology and Immunology, University of Dentistry of São José dos Campos/UNESP. The quantification of gene expression will be related to the production of hydrolytic enzymes (SAP5, PLB and LIP) in clinical samples and the reference strain using the RT-PCR assay in real time. The presence and expression of the genes will be analyzed before and after photodynamic therapy. For photodynamic therapy will be used laser-aluminum-gallium arsenide low power (red visible, 660 nm) as light source and the methylene blue to 300 µM, as a photosensitizer. They will be appraised four experimental groups, in triplicate, for each strain: a) F+L+: sensitization with methylene blue and laser irradiation; b) F+L-: only treatment with methylene blue; c) F-L+: only laser irradiation and d) F-L-: control group, without sensitization with methylene blue and light absence. The results will be analyzed statistically (p<0.05).