EVALUATION OF TEMPORAL EXPRESSION OF GENES SAP5, SAP9, PLB1, PLB2, LIP9 LIP10 IN INTERACTIONS BETWEEN SPECIES C. albicans, C. tropicalis, C. glabrata and C. krusei.

• Grant number: 12/15250-0
• Support Opportunities: Scholarships in Brazil - Doctorate
• Effective date (Start): November 01, 2012
• Effective date (End): January 31, 2015
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Antonio Olavo Cardoso Jorge
• Grantee: Patrícia Pimentel de Barros
• Host Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil

Abstract

The broad spectrum of candidiasis and clinical relevance of infections propel the research that aim to clarifying the pathogenic mechanisms and identification of virulence factors of Candida albicans. Therefore, the aim of this study is to evaluate the interactions between the species C. albicans and non-albicans species, time through the quantification of genes related to production of hydrolytic enzymes, protease (SAP), phospholipase B (PLB), lipase (IPL) in clinical samples and samples isolated reference model in the biofilm. Will be used six clinical strains and two from the association between C. albicans / C. krusei, C. albicans / C. tropicalis and C. albicans /C. glabrata. All strains are from the Laboratory of Microbiology and Immunology, Faculty of Dentistry of São José dos Campos / UNESP and has been isolated from saliva and oropharyngeal candidiasis lesions in HIV-positive patients of the Emilio Ribas Institute of Infectious Diseases. The quantification of gene expression is performed in the following time intervals (3h, 12h and 24h) of biofilms heterotypic by testing RT-PCR in real time. The genes will be quantified both in heterotypic biofilms formed by the reference samples: C. albicans (ATCC 18804) with C. glabrata (ATCC 90030), C. albicans (ATCC 18804) with C. tropicalis (ATCC 13803) and C. albicans (ATCC 18804) with C. krusei (ATCC 6258) in relation formed by strains isolated from clinical samples of saliva and lesions of patients with HIV. The presence and quantity of the genes analyzed during the time intervals for heterotypic biofilm formation will be compared in different types of associations, providing a better understanding of the pathogenicity of strains of Candida when combined together in an experimental model.

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE MELLO, MIRIAN MARCOLAN; DE BARROS, PATRICIA PIMENTEL; BERNARDES, RENATA DE CASSIA; ALVES, SILVIO RUBENS; RAMANZINI, NAIARA PIRES; ALVES FIGUEIREDO-GODOI, LIVIA MARA; CHIPOLETTI PRADO, ANA CAROLINA; CARDOSO JORGE, ANTONIO OLAVO; JUNQUEIRA, JULIANA CAMPOS. Antimicrobial photodynamic therapy against clinical isolates of carbapenem-susceptible and carbapenem-resistant Acinetobacter baumannii. Lasers in Medical Science, v. 34, n. 9, p. 1755-1761, DEC 2019. (14/03937-6, 12/15250-0)

DE BARROS, PATRICIA PIMENTEL; FREIRE, FERNANDA; ROSSONI, RODNEI DENNIS; JUNQUEIRA, JULIANA CAMPOS; CARDOSO JORGE, ANTONIO OLAVO. Candida krusei and Candida glabrata reduce the filamentation of Candida albicans by downregulating expression of HWP1 gene. Folia Microbiologica, v. 62, n. 4, p. 317-323, JUL 2017. (11/15194-0, 12/15250-0)

DE BARROS, PATRICIA PIMENTEL; ROSSONI, RODNEI DENNIS; RIBEIRO, FELIPE DE CAMARGO; JUNQUEIRA, JULIANA CAMPOS; CARDOSO JORGE, ANTONIO OLAVO. Temporal Profile of Biofilm Formation, Gene Expression and Virulence Analysis in Candida albicans Strains. Mycopathologia, v. 182, n. 3-4, p. 285-295, APR 2017. (12/15250-0, 12/02184-9, 11/15194-0)

BARROS, PATRICIA PIMENTEL; RIBEIRO, FELIPE CAMARGO; ROSSONI, RODNEI DENNIS; JUNQUEIRA, JULIANA CAMPOS; CARDOSO JORGE, ANTONIO OLAVO. Influence of Candida krusei and Candida glabrata on Candida albicans gene expression in in vitro biofilms. ARCHIVES OF ORAL BIOLOGY, v. 64, p. 92-101, APR 2016. (11/15194-0, 12/15250-0)

DE BARROS, PATRICIA PIMENTEL; ROSSONI, RODNEI DENNIS; FREIRE, FERNANDA; RIBEIRO, FELIPE DE CAMARGO; DAS CHAGAS LOPES, LUCAS ALEXANDRE; JUNQUEIRA, JULIANA CAMPOS; CARDOSO JORGE, ANTONIO OLAVO. Candida tropicalis affects the virulence profile of Candida albicans: an in vitro and in vivo study. PATHOGENS AND DISEASE, v. 76, n. 2, MAR 2018. (13/25181-8, 15/09770-9, 12/15250-0)