The broad spectrum of candidiasis and clinical relevance of infections propel the research that aim to clarifying the pathogenic mechanisms and identification of virulence factors of Candida albicans. Therefore, the aim of this study is to evaluate the interactions between the species C. albicans and non-albicans species, time through the quantification of genes related to production of hydrolytic enzymes, protease (SAP), phospholipase B (PLB), lipase (IPL) in clinical samples and samples isolated reference model in the biofilm. Will be used six clinical strains and two from the association between C. albicans / C. krusei, C. albicans / C. tropicalis and C. albicans /C. glabrata. All strains are from the Laboratory of Microbiology and Immunology, Faculty of Dentistry of São José dos Campos / UNESP and has been isolated from saliva and oropharyngeal candidiasis lesions in HIV-positive patients of the Emilio Ribas Institute of Infectious Diseases. The quantification of gene expression is performed in the following time intervals (3h, 12h and 24h) of biofilms heterotypic by testing RT-PCR in real time. The genes will be quantified both in heterotypic biofilms formed by the reference samples: C. albicans (ATCC 18804) with C. glabrata (ATCC 90030), C. albicans (ATCC 18804) with C. tropicalis (ATCC 13803) and C. albicans (ATCC 18804) with C. krusei (ATCC 6258) in relation formed by strains isolated from clinical samples of saliva and lesions of patients with HIV. The presence and quantity of the genes analyzed during the time intervals for heterotypic biofilm formation will be compared in different types of associations, providing a better understanding of the pathogenicity of strains of Candida when combined together in an experimental model.
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