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Comparative analysis of Candida albicans isolated from complete denture wearers with and without HIV diagnosis

Grant number: 19/10299-0
Support type:Scholarships in Brazil - Master
Effective date (Start): December 01, 2019
Effective date (End): April 30, 2021
Field of knowledge:Biological Sciences - Microbiology - Applied Microbiology
Principal researcher:Helena de Freitas Oliveira Paranhos
Grantee:Filipe Santos Ferreira Mendes
Home Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

The Candida albicans strain is a naturally occurring fungus found in the oral cavity commensal microbiota and can assume pathogenic characteristics when there are changes in its environment, such as changes in the microbiota or decreased host immunity, and one of the main risk groups are people living with HIV. The aim of this study will be to compare the incidence, virulence and morphology in vitro of strains of clinically isolated C. albicans from patients using total removable dentures with denture-related stomatitis in patients with and without HIV diagnosis. For the analysis of the strains, biofilm of total removable prostheses will be collected by the standardized method of ultrasonification in a university hospital and a dental school. The tests will be: Identification of Candida species isolated from the biofilm of the total prosthesis, using chromogenic and polymerase chain reaction (PCR) methods; Virulence factors: Biofilm formation capacity by measuring colony forming units (CFU/mL); cellular metabolism by tetrazolium salt metabolism; identification of possibly modulatory genes involved in proteinase, lipase and phospholipase synthesis by real-time amplification reaction (qPCR) tests; Proteinase and Phospholipase production and hyphae production. The study will be conducted in two dental care centers, between 2019 and 2020. The data obtained will be initially analyzed for normality and homoscedasticity, and then submitted to the indicated statistical tests, using a confidence level of 95% (± = 0,05). (AU)

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