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| Author(s): |
Filipe Santos Ferreira Mendes
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | Ribeirão Preto. |
| Institution: | Universidade de São Paulo (USP). Faculdade de Odontologia de Ribeirão Preto (PCARP/BC) |
| Defense date: | 2021-10-22 |
| Examining board members: |
Helena de Freitas Oliveira Paranhos;
Alan Grupioni Lourenço;
Carlos Henrique Gomes Martins;
Cláudia Helena Lovato da Silva
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| Advisor: | Helena de Freitas Oliveira Paranhos |
| Abstract | |
Denture-related stomatitis (DRS) is an inflammation of the mucosa of the supporting tissue of complete dentures, frequent in HIV+ individuals, that of main factors is the colonization by Candida spp. The aim of this study was to evaluate the prevalence, virulence and morphology of Candida spp. isolated from biofilms of complete denture wearers with denture-related stomatitis with and without HIV diagnosis. Fifty-five users of complete dentures diagnosed with DRS were divided in two groups: Experimental (HIV+) and Control (HIV-). Biofilm was collected using a standardized method of ultrasonification of prostheses. The prevalence was evaluated through identification by the chromogenic method CHROMagar Candida, and confirmation of isolates by polymerase chain reaction (PCR). Virulence factors were measured by the biofilm formation capacity by counting colony-forming units (CFU/mL), biofilm metabolism by tetrazolium salt metabolization, and the proteinase and phospholipase production by a fluorimetric kit. Morphology was verified using the hyphae inducing test. Health data were collected using a form and, for the experimental group, the correlation between the ability to form biofilm with DRS, viral load and CD4+T cells count were evaluated; as well as the relationship between DRS with viral load and CD4+T lymphocyte count. Virulence and morphology factors were compared by Student\'s T test, except for phospholipase (C. albicans) and proteinase (non-albicans) production, which were compared by Mann-Whitney U test. For health data, Spearman\'s and Fisher\'s exact tests were used. A significance level of 5% (α = 0.05) was used. The prevalence results included 55 patients (22 experimental and 33 control), a total of 63 samples of Candida spp. isolated, being 28 strains of Candida albicans and 36 non-albicans strains. There was no significant difference between groups regarding baseline CFU/mL count, biofilm formation capacity, cell metabolism and phospholipase production. There was a higher production of proteinase for C. albicans in the control (p=0.031) and for non-albicans in the experimental (p=0.016). Regarding the morphology, there was no significant difference between the groups (Control and experimental) (C. albicans - p=0.860; non-albicans - p=0.501). As for health data, for the experimental group, viral load was undetectable in 13 patients (59%), the CD4+T count had a mean of 565 cells per mm3 , there was a moderate negative correlation between the CFU count/mL in baseline for non-albicans and DRS classification (ρ= -0.592, p=0.020) and there was no relationship between DRS and viral load (p=0.1772), as well as between DRS and CD4+ T count (p=0.3624). It was concluded that C. albicans was the most isolated species, that there was no difference regarding Candida spp. from complete denture wearers with ERP, with and without HIV diagnosis for virulence factors (except for proteinase production) and morphology. (AU) | |
| FAPESP's process: | 19/10299-0 - Comparative analysis of Candida albicans isolated from complete denture wearers with and without HIV diagnosis |
| Grantee: | Filipe Santos Ferreira Mendes |
| Support Opportunities: | Scholarships in Brazil - Master |