Leishmania infection and neuroinflammation: Specific chemokine profile and absence of parasites in the brain of naturally-infected dogs

Autor(es)

Melo, Guilherme D. ^[1] ; Silva, Jose Eduardo S. ^[1] ; Grano, Femanda G. ^[1] ; Souza, Milena S. ^[2] ; Machado, Gisele F. ^[1]

Afiliação do(s) autor(es)

^[1] UNESP Univ Estadual Paulista, Fac Med Vet, Lab Patol Aplicada LApap, Sao Paulo - Brazil

^[2] UNESP Univ Estadual Paulista, Fac Med Vet, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Neuroimmunology; v. 289, p. 21-29, DEC 15 2015.
• Citações Web of Science: 6

Resumo

Visceral leishmaniasis is a chronic disease caused by Leishmania infantum. We aimed to detect the parasite in the brain of fifteen naturally-infected dogs using in situ hybridization and immunohistochemistry, and the gene expression of selected chemokines by RT-qPCR. We detected no parasite in the brain, but perivascular deposition of parasite DNA and IgG in the choroid plexus. We noticed up-regulation of CCL-3, CCL-4 and CCL-5, coherent with T lymphocyte accumulation, stating the brain as a pro-inflammatory environment. Indeed, not necessarily the parasite itself, but rather its DNA seems to act as a trigger to promote brain inflammation during visceral leishmaniasis. (C) 2015 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 12/10415-0 - Neuropatogenia da leishmaniose visceral canina: investigação de mediadores inflamatórios
• Beneficiário: Guilherme Dias de Melo
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 12/23950-1 - Neuropatogenia da leishmaniose visceral canina: investigação de mediadores inflamatórios
• Beneficiário: Gisele Fabrino Machado
• Modalidade de apoio: Auxílio à Pesquisa - Regular