Antioxidant metabolism in coffee (Coffea arabica L.) plants in response to nitrogen supply

Autor(es)

Reis, Andre R. ^[1] ; Favarin, Jose L. ^[2] ; Gratao, Priscila L. ^[3] ; Capaldi, Flavia R. ^[4] ; Azevedo, Ricardo A. ^[4]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista UNESP, Engn Biossistemas, Tupa, SP - Brazil

^[2] Univ Sao Paulo, Escola Super Agr Luiz de Queiroz, Dept Agr, BR-13418900 Piracicaba, SP - Brazil

^[3] Univ Estadual Paulista UNESP, Fac Ciencias Agr & Vet, Dept Biol Aplicada Agr, BR-14884900 Jaboticabal, SP - Brazil

^[4] Univ Sao Paulo, Escola Super Agr Luiz de Queiroz, Dept Genet, BR-13418900 Piracicaba, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: THEORETICAL AND EXPERIMENTAL PLANT PHYSIOLOGY; v. 27, n. 3-4, p. 203-213, DEC 2015.
• Citações Web of Science: 8

Resumo

Nitrogen (N) is the main element required for plant development. N fertilization interferes directly in N content in tissues, antioxidant systems, chlorophyll content and photosynthesis. We investigated the action of three levels of N (0, 150 and 300 kg N ha(-1)) in coffee (Coffea arabica L.) cv. Catuai Vermelho IAC 44 leaves of plants during distinct fruit development stages (from pinhead drop to dried fruit). Leaf N content, net photosynthesis, chlorophyll content, total soluble protein and the activities of catalase, superoxide dismutase, guaiacol peroxidase and ascorbate peroxidase were analyzed. Leaf N content, net photosynthesis, chlorophyll and protein content increased significantly with N supply (N deficient plants exhibited visual symptoms of chlorosis). Antioxidant enzymes showed increased specific activities during fruit development, and decreases with N fertilization, being higher in absence of N. We identified two bands of Mn-SOD with increased activities and one of Fe-SOD, but they did not exhibit high SOD activity remaining essentially constant. Curiously, Cu/Zn-SOD isoenzymes were not detected, despite the fact that they are frequently abundant in plants. A relationship between N fertilization and antioxidant enzyme activities were founded in coffee leaves during fruit development indicating high activity of enzymatic antioxidant system during the coffee fruit ripening stage. (AU)

• Processo FAPESP: 06/54552-0 - Caracteristicas fisiologicas e bioquimicas da plnata e sementes de cafeeiro em funcao das doses de nitrogenio.
• Beneficiário: André Rodrigues dos Reis
• Modalidade de apoio: Bolsas no Brasil - Mestrado