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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Antioxidant metabolism in coffee (Coffea arabica L.) plants in response to nitrogen supply

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Author(s):
Reis, Andre R. [1] ; Favarin, Jose L. [2] ; Gratao, Priscila L. [3] ; Capaldi, Flavia R. [4] ; Azevedo, Ricardo A. [4]
Total Authors: 5
Affiliation:
[1] Univ Estadual Paulista UNESP, Engn Biossistemas, Tupa, SP - Brazil
[2] Univ Sao Paulo, Escola Super Agr Luiz de Queiroz, Dept Agr, BR-13418900 Piracicaba, SP - Brazil
[3] Univ Estadual Paulista UNESP, Fac Ciencias Agr & Vet, Dept Biol Aplicada Agr, BR-14884900 Jaboticabal, SP - Brazil
[4] Univ Sao Paulo, Escola Super Agr Luiz de Queiroz, Dept Genet, BR-13418900 Piracicaba, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: THEORETICAL AND EXPERIMENTAL PLANT PHYSIOLOGY; v. 27, n. 3-4, p. 203-213, DEC 2015.
Web of Science Citations: 8
Abstract

Nitrogen (N) is the main element required for plant development. N fertilization interferes directly in N content in tissues, antioxidant systems, chlorophyll content and photosynthesis. We investigated the action of three levels of N (0, 150 and 300 kg N ha(-1)) in coffee (Coffea arabica L.) cv. Catuai Vermelho IAC 44 leaves of plants during distinct fruit development stages (from pinhead drop to dried fruit). Leaf N content, net photosynthesis, chlorophyll content, total soluble protein and the activities of catalase, superoxide dismutase, guaiacol peroxidase and ascorbate peroxidase were analyzed. Leaf N content, net photosynthesis, chlorophyll and protein content increased significantly with N supply (N deficient plants exhibited visual symptoms of chlorosis). Antioxidant enzymes showed increased specific activities during fruit development, and decreases with N fertilization, being higher in absence of N. We identified two bands of Mn-SOD with increased activities and one of Fe-SOD, but they did not exhibit high SOD activity remaining essentially constant. Curiously, Cu/Zn-SOD isoenzymes were not detected, despite the fact that they are frequently abundant in plants. A relationship between N fertilization and antioxidant enzyme activities were founded in coffee leaves during fruit development indicating high activity of enzymatic antioxidant system during the coffee fruit ripening stage. (AU)