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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Development of a qPCR for Leifsonia xyli subsp xyli and quantification of the effects of heat treatment of sugarcane cuttings on Lxx

Texto completo
Autor(es):
Carvalho, G. [1] ; da Silva, T. G. E. R. [2] ; Munhoz, A. T. [2] ; Monteiro-Vitorello, C. B. [1] ; Azevedo, R. A. [1] ; Melotto, M. [3] ; Camargo, L. E. A. [2]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Escola Super Agr Luiz de Queiroz, Dept Genet, BR-13418900 Sao Paulo - Brazil
[2] Univ Sao Paulo, Escola Super Agr Luiz de Queiroz, Dept Fitopatol & Nematol, BR-13418900 Sao Paulo - Brazil
[3] Univ Calif Davis, Dept Plant Sci, Coll Agr & Environm Sci, Davis, CA 95616 - USA
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: CROP PROTECTION; v. 80, p. 51-55, FEB 2016.
Citações Web of Science: 8
Resumo

The main control practice of Leifsonia xyli subsp. xyli (Lxx) in sugarcane is to heat-treat cane cuttings used as planting material in an attempt to eradicate the bacterium. A real time quantitative PCR (qPCR) protocol specific for Lxx was developed to assess the effectiveness of this practice. Primers were designed from the sequence of an Lxx-specific gene and detected as few as 10(-5) ng of Lxx DNA in 100 ng of plant DNA. Two experiments were conducted to quantify Lxx titers in plants of the varieties SP80-3280 and SP70-3370 originated from cuttings treated or not by immersion in hot water at 52 degrees C for 30 min. In the first experiment, cuttings were collected from plant canes with low Lxx titers whereas in the second they were collected from first-ratoon canes with higher titers. Lxx was quantified in leaves by qPCR 90 days after planting and was detected in 50-90% of the plants at variable titers, indicating that the 52 degrees C hot water treatment for 30 min was not effective in eradicating Lxx from all plants. However, in the second experiment the bacterial population was reduced, as the median number of Lxx cells was lower compared to the non-treated control. In the case of SP70-3370, the treatment also reduced the number of Lxx-infected plants considering the pooled data of the two experiments. The results indicated that although the 52 degrees C hot water treatment for 30 min did not completely eliminate Lxx, it can be used to reduce the pathogen population in plants propagated from canes with high Lxx titers (C) 2015 Elsevier Ltd. All rights reserved. (AU)

Processo FAPESP: 08/56260-2 - Functional'omics of the ratoon stunting disease of sugarcane
Beneficiário:Luis Eduardo Aranha Camargo
Linha de fomento: Auxílio à Pesquisa - Programa BIOEN - Temático
Processo FAPESP: 08/56239-3 - Análise do proteoma e do sistema antioxidante de cana-de-açúcar em resposta a colonização por Leifsonia xyli subsp.wyli, agente causal do raquistismo-das-soqueiras
Beneficiário:Giselle de Carvalho
Linha de fomento: Bolsas no Brasil - Doutorado