The effects of freeze/thawing on the function and phenotype of CD4(+) lymphocyte subsets in normal individuals and patients with systemic lupus erythematosus

Autor(es)

Ferreira, Vanessa da Silva ^[1] ; Mesquita, Fernanda Vieira ^[1] ; Mesquita Junior, Danilo ^[1] ; Coelho Andrade, Luis Eduardo ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Div Rheumatol, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: CRYOBIOLOGY; v. 71, n. 3, p. 507-510, DEC 2015.
• Citações Web of Science: 3

Resumo

Several studies report on lymphocyte phenotypic and functional abnormalities in Systemic Lupus Erythematosus (SLE). Freezing and thawing may alter functional and phenotypic properties of cells. We assessed the effect of the freezing/thawing process (FIT) on Th1 (CD3(+)CD4(+)CCR4(-)CXCR3(+)CCR5(+)), Th2 (CD3(+)CD4(+)CCR5(-)CXCR3(-)CCR4(+)), Th17 (CD3(+)CD4(+)CCR6(+)CD161(+)), and Treg (CD3(+)CD4(+)CD25(high)CD127(-)) cell cultures in healthy controls and SLE patients. F/T was associated with decreased frequency of Th2 and Th17 cells in cultures from SLE patients but not from controls. FIT was also associated with increased frequency of apoptotic cells, as measured by annexin V labeling, in all T cell subtypes analyzed, as well as increased cell proliferation, as measured by Ki-67 labeling, in all cells except Th1 from SLE patients. Thus, F/T can have differentiated effects on T lymphocyte subtypes from SLE patients and controls, and can have significant effects on cell death and proliferation. These findings should be carefully considered when designing and interpreting studies on functional and phenotypic aspects of T lymphocytes in SLE. (C) 2015 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 09/12930-7 - Apoptose, células Th1 e células Th2 no lúpus eritematoso sistêmico
• Beneficiário: Vanessa da Silva Ferreira
• Modalidade de apoio: Bolsas no Brasil - Mestrado