Differential colonization patterns of Xylella fastidiosa infecting citrus genotypes

Autor(es)

Niza, B. ^{[1, 2]} ; Coletta-Filho, H. D. ^[1] ; Merfa, M. V. ^{[1, 2]} ; Takita, M. A. ^[1] ; de Souza, A. A. ^[1]

Afiliação do(s) autor(es)

^[1] Inst Agron Campinas, Ctr Citricultura Sylvio Moreira, Sao Paulo - Brazil

^[2] Univ Estadual Campinas, Inst Biol, Dept Genet Evolucao & Bioagentes, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLANT PATHOLOGY; v. 64, n. 6, p. 1259-1269, DEC 2015.
• Citações Web of Science: 15

Resumo

Xylella fastidiosa is a phytopathogenic bacterium that causes disease in many different crops worldwide. In Brazil, X. fastidiosa subsp. pauca causes citrus variegated chlorosis (CVC), which is a disease responsible for economic losses in the citrus agribusiness. Variable host responses to bacterial colonization and disease development have been observed. This work studies the colonization processes of a pathogenic GFP-labelled X. fastidiosa citrus strain in sweet orange (susceptible) and tangor (resistant) parents and two resulting hybrids that exhibited contrasting responses to CVC. Xylella fastidiosa showed increased populations and movement in the susceptible genotypes, but slower compared to other hosts such as grapevine. Scanning electron microscopy revealed that the predominant pitted stem morphology in citrus makes the bacterial movement difficult. In susceptible genotypes X. fastidiosa can move from the primary to the secondary xylem, whilst it is confined to the primary xylem in resistant plants. Associated with this is an induction of lignification that occurs earlier in the resistant genotypes when in the presence of the pathogen, and represents a genetic mechanism that leads to formation of a physical barrier, impairing bacterial colonization. (AU)

• Processo FAPESP: 10/50840-7 - Estudo funcional de genes de citrus reticulata associados a resistencia a clorose variegada dos citros atraves da superexpressao em citrus sinensis e arabdopsis thaliana.
• Beneficiário: Alessandra Alves de Souza
• Linha de fomento: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/14307-5 - Estudo da interação Xylella fastidiosa-hospedeiro: efeito do movimento e colonização in planta e transmissão pelo inseto vetor
• Beneficiário: Bárbara Caroline Niza Silva
• Linha de fomento: Bolsas no Brasil - Mestrado
• Processo FAPESP: 08/57909-2 - Plataforma genômica aplicada ao melhoramento de citros
• Beneficiário: Marcos Antonio Machado
• Linha de fomento: Auxílio à Pesquisa - Temático
• Processo FAPESP: 13/17485-7 - Estudo funcional de genes associados à defesa de plantas a fitopatógenos: foco no controle de Xylella fastidiosa, agente causal da clorose variegada dos citros
• Beneficiário: Alessandra Alves de Souza
• Linha de fomento: Auxílio à Pesquisa - Regular