Altered microRNome Profiling in Statin-Induced HepG2 Cells: A Pilot Study Identifying Potential new Biomarkers Involved in Lipid-Lowering Treatment

Autor(es)

Zambrano, Tomas ^{[1, 2]} ; Hirata, Rosario D. C. ^[1] ; Hirata, Mario H. ^[1] ; Cerda, Alvaro ^{[1, 2]} ; Salazar, Luis A. ^{[2, 3]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Pharmaceut Sci, Sao Paulo - Brazil

^[2] Univ La Frontera, Ctr Mol Biol & Pharmacogenet Sci & Technol Biores, Temuco - Chile

^[3] Univ La Frontera, Ctr Biol Mol & Farmacogenet, Dept Ciencias Basicas, Fac Med, Temuco - Chile

• Tipo de documento: Artigo Científico
• Fonte: CARDIOVASCULAR DRUGS AND THERAPY; v. 29, n. 6, p. 509-518, DEC 2015.
• Citações Web of Science: 9

Resumo

Purpose Statins are widely prescribed drugs to manage hypercholesterolemia. Despite they are considered effective lipid-lowering agents, significant inter-individual variability has been reported in relation to drug response. Among the reasons explaining this variation, genetic factors are known to partially contribute. Nonetheless, poor evidence exists regarding epigenetic factors involved. Methods We investigated if atorvastatin can modulate the cholesterol related miR-33 family. Furthermore, we analyzed the microRNA expression profiles in HepG2 cells treated for 24 hours with atorvastatin or simvastatin using a microarray platform. Results Our results indicate that atorvastatin does not influence the expression of the miR-33 family. In addition, microarray examination revealed that atorvastatin modulated thirteen miRs, whilst simvastatin only affected two miRs. All significantly modulated miRs after simvastastin therapy were also modulated by atorvastatin. In addition, four novel miRs with previously unreported functions were identified as statin-modulated. Conclusion We identified several novel miRs affected by statin treatment. Additional research is needed to determine the biological significance of differentially expressed miRs identified in statins-induced HepG2 cells. (AU)

• Processo FAPESP: 11/21967-1 - Papel de estatinas na modulação de microRNAs em células mononucleares de pacientes hipercolesterolêmicos
• Beneficiário: Rosario Dominguez Crespo Hirata
• Modalidade de apoio: Auxílio à Pesquisa - Regular