Effects of ammonia stress in the Amazon river shrimp Macrobrachium amazonicum (Decapoda, Palaemonidae)

Autor(es)

Pinto, Marcelo R. ^[1] ; Lucena, Malson N. ^[1] ; Faleiros, Rogerio Oliveira ^[2] ; Almeida, Eduardo Alves ^[3] ; McNamara, John C. ^[2] ; Leone, Francisco A. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Quim, Ave Bandeirantes 3900, BR-14040901 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Biol, BR-14040901 Ribeirao Preto, SP - Brazil

^[3] Univ Estadual Paulista, Campus Sao Jose Rio Preto, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: AQUATIC TOXICOLOGY; v. 170, p. 13-23, JAN 2016.
• Citações Web of Science: 19

Resumo

We evaluate the effects of total ammonia nitrogen-N (TAN) exposure for 72 h on (Na+,K+)- and V(H+)-ATPase activities and on their subunit expressions in gills of the diadromous freshwater shrimp Macrobrachium amazonicum. Specific (Na+,K+)- and V(H+)-ATPase activities increased roughly 1.5- to 2 fold, respectively, after exposure to 2.0 mmol L-1 TAN. Quantitative RT-PCR analyses revealed a 2.5-fold increase in V(H+)-ATPase B subunit mRNA expression while (Na+,K+)-ATPase alpha-subunit expression was unchanged. Immunohistochemical analyses of the gill lamellae located the (Na+,K+)-ATPase throughout the intralamellar septal cells, independently of TAN concentration, while the V(H+)-ATPase was located in both the apical pillar cell flanges and pillar cell bodies. Systemic stress parameters like total hemocyte count decreased by 30% after exposure to 2.0 mmol L-1 TAN, accompanied by increased activities of the oxidative stress enzymes superoxide dismutase, glutathione reductase and glucose-6-phosphate dehydrogenase in the gills. The stress responses of M. amazonicum to elevated TAN include increases in gill (Na+,K+)- and V(H+)-ATPase activities that are accompanied by changes in oxidative stress enzyme activities, immune system effects and an increase in gill V(H+)-ATPase gene expression. These findings likely underpin physiological effects in a crustacean like M. amazonicum that exploits multiple ecosystems during its life cycle, as well as under culture conditions that may significantly impact shrimp production by the aquaculture industry. (C) 2015 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 13/22605-1 - Caracterização cinética e molecular da (Na+,K+)-ATPase do tecido branquial do caranguejo Cardisoma guanhumi (Latreille)
• Beneficiário: Francisco de Assis Leone
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 10/17534-0 - Papel da (Na,K)-ATPase na osmorregulação e excreção de amônio em crustáceos eurialinos: identificação e efeitos do FXYD2 na regulação da (Na,K)-ATPase branquial do siri Callinectes danae
• Beneficiário: Francisco de Assis Leone
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/15776-9 - Expressão de RNAm e atividade enzimática de transportadores iônicos nas brânquias de caranguejos do gênero Uca, os chama-marés: capacidade osmorregulatória e a ocupação de nichos osmoticamente distintos
• Beneficiário: Rogério Oliveira Faleiros
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 13/24252-9 - O papel da (Na+,K+)-ATPase na capacidade osmorregulatória do caranguejo Ucides cordatus: uma abordagem bioquímica e molecular
• Beneficiário: Malson Neilson de Lucena
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado