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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Effects of ammonia stress in the Amazon river shrimp Macrobrachium amazonicum (Decapoda, Palaemonidae)

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Author(s):
Pinto, Marcelo R. [1] ; Lucena, Malson N. [1] ; Faleiros, Rogerio Oliveira [2] ; Almeida, Eduardo Alves [3] ; McNamara, John C. [2] ; Leone, Francisco A. [1]
Total Authors: 6
Affiliation:
[1] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Quim, Ave Bandeirantes 3900, BR-14040901 Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Biol, BR-14040901 Ribeirao Preto, SP - Brazil
[3] Univ Estadual Paulista, Campus Sao Jose Rio Preto, Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: AQUATIC TOXICOLOGY; v. 170, p. 13-23, JAN 2016.
Web of Science Citations: 20
Abstract

We evaluate the effects of total ammonia nitrogen-N (TAN) exposure for 72 h on (Na+,K+)- and V(H+)-ATPase activities and on their subunit expressions in gills of the diadromous freshwater shrimp Macrobrachium amazonicum. Specific (Na+,K+)- and V(H+)-ATPase activities increased roughly 1.5- to 2 fold, respectively, after exposure to 2.0 mmol L-1 TAN. Quantitative RT-PCR analyses revealed a 2.5-fold increase in V(H+)-ATPase B subunit mRNA expression while (Na+,K+)-ATPase alpha-subunit expression was unchanged. Immunohistochemical analyses of the gill lamellae located the (Na+,K+)-ATPase throughout the intralamellar septal cells, independently of TAN concentration, while the V(H+)-ATPase was located in both the apical pillar cell flanges and pillar cell bodies. Systemic stress parameters like total hemocyte count decreased by 30% after exposure to 2.0 mmol L-1 TAN, accompanied by increased activities of the oxidative stress enzymes superoxide dismutase, glutathione reductase and glucose-6-phosphate dehydrogenase in the gills. The stress responses of M. amazonicum to elevated TAN include increases in gill (Na+,K+)- and V(H+)-ATPase activities that are accompanied by changes in oxidative stress enzyme activities, immune system effects and an increase in gill V(H+)-ATPase gene expression. These findings likely underpin physiological effects in a crustacean like M. amazonicum that exploits multiple ecosystems during its life cycle, as well as under culture conditions that may significantly impact shrimp production by the aquaculture industry. (C) 2015 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 11/15776-9 - MRNA expression and ion transporter activity in fiddler crab (Uca) gills: does osmoregulatory capability underlie the occupation of distinct osmotic niches?
Grantee:Rogério Oliveira Faleiros
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 13/24252-9 - The (Na+,K+)-ATPase in osmoregulatory capacity in crab Ucides cordatus: biochemical and molecular view
Grantee:Malson Neilson de Lucena
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 13/22605-1 - Kinetic and molecular characterization of gill tissue (Na+,K+)-ATPase from Cardisoma guanhumi (Latreille)crab
Grantee:Francisco de Assis Leone
Support type: Regular Research Grants
FAPESP's process: 10/17534-0 - Role of (Na,K)-ATPase in osmoregulation and excretion of ammonium ions in euryhaline crustaceans: identification and influence of FXYD2 on the regulation of gill (Na,K)-ATPase of the crab Callinectes danae
Grantee:Francisco de Assis Leone
Support type: Regular Research Grants