Embryonic development of the Amazonian shrimp Macrobrachium amazonicum (Heller, 1862)(CRUSTACEA, Decapoda, Palaemonidae)

• Grant number: 10/14576-3
• Support Opportunities: Scholarships in Brazil - Master
• Start date: March 01, 2011
• End date: February 29, 2012
• Field of knowledge: Biological Sciences - Morphology - Embryology
• Principal Investigator: Irene Bastos Franceschini Vicentini
• Grantee: Ángel Andrés Arias Vigoya
• Host Institution: Faculdade de Ciências (FC). Universidade Estadual Paulista (UNESP). Campus de Bauru. Bauru , SP, Brazil

Abstract

Embryonic development in decapod crustaceans display a wide variety of patterns, however, detailed studies describing the different stages of embryonic development of several species of the genus Macrobrachium have not yet been developed, including the Amazonian shrimp, Macrobrachium amazonicum. A study aiming to establish the stages of embryonic development of this species, with emphasis on analysis of morphological characteristics. Will be used 10 tanks with 80 liters in volume, coupled with biological filters, and under controlled conditions of aeration, temperature, salinity and photoperiod. Ovigerous females are monitored daily to remove samples of 10 eggs. Embryogenesis is characterized by daily staging system. The eggs are examined under a stereomicroscope and light microscope in two phases of collection, the first every 24 hours for standardization of embryogenesis, and the second at two hours each, with specific focus to previous periods that exhibited severe morphological changes. The eggs will be fixed in alcoholic Bouin's solution and later preserved in 70% alcohol. The samples will be included in historesin, sectioned (sections of 3 microns) and stained with toluidine blue and hematoxylin / eosin. The analysis and photo documentation will be held in Olympus BX50 light microscope. The Perkins index is estimated and biometric measurements are made of eggs and embryos, with the use of ocular micrometer. (AU)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

VIGOYA, Ángel Andrés Arias. Desenvolvimento embrionário do camarão-da-amazônia Macrobrachium amazonicum (Heller, 1862) (Crustacea, Decapoda, Palaemonidae). 2012. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Ciências Agrárias e Veterinárias. Jaboticabal Jaboticabal.