Development of on-line molecularly imprinted solid phase extraction-liquid chromatography-mass spectrometry for triazine analysis in corn samples

Autor(es)

Andrade, Felipe Nascimento ^[1] ; Domingues Nazario, Carlos Eduardo ^[1] ; Santos-Neto, Alvaro Jose ^[1] ; Lancas, Fernando Mauro ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Chem Sao Carlos, Postal Code 780, BR-13560970 Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ANALYTICAL METHODS; v. 8, n. 5, p. 1181-1186, 2016.
• Citações Web of Science: 18

Resumo

A highly selective method for the analysis of triazine herbicides in corn samples based on molecularly imprinted solid phase extraction (MISPE) has been developed. Molecularly imprinted polymers (MIPs) were synthesized by precipitation polymerization using atrazine as a template, methacrylic acid as a functional monomer, ethylene glycol dimethacrylate as a crosslinker, and 2,2'-azobis-isobutrynitrile as an initiator. MISPE was developed for the on-line and automated enrichment of atrazine, simazine, terbutryn, simetryn and ametryn from corn sample extracts. High-performance liquid chromatography and time-of-flight mass spectrometry were used for the separation and confident determination of the herbicides. The limits of detection and quantitation of the proposed method were set to be 1.6-3.3 mu g kg(-1) and 5.0-10.0 mu g kg(-1). The method was successfully applied for the analysis of five types of corns and the recoveries of the triazines from the spiked samples ranged from 80.2 to 119.1%. (AU)

• Processo FAPESP: 11/09898-4 - Desenvolvimento de polímeros molecularmente impressos e seu uso para o preparo de amostras e análise de triazinas e sulfoniluréias em milho utilizando cromatografia líquida de alta eficiência (HPLC).
• Beneficiário: Felipe Nascimento Andrade
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 14/07347-9 - Desenvolvimento de novos materiais e dispositivos para técnicas miniaturizadas de preparo de amostras na área de segurança alimentar
• Beneficiário: Fernando Mauro Lanças
• Modalidade de apoio: Auxílio à Pesquisa - Regular