Penetration of Resin-Based Materials Into Initial Erosion Lesion: A Confocal Microscopic Study

Autor(es)

Ionta, Franciny Querobim ^[1] ; Boteon, Ana Paula ^[2] ; Moretto, Marcelo Juliano ^[3] ; Bim Junior, Odair ^[4] ; Honorio, Heitor Marques ^[1] ; Silva, Thiago Cruvinel ^[1] ; Wang, Linda ^[4] ; Rios, Daniela ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, FOB, Bauru Sch Dent, Dept Pediat Dent Orthodont & Publ Hlth, Alameda Dr Octavio Pinheiro Brisolla 9-75, BR-17012101 Sao Paulo - Brazil

^[2] Univ Sao Paulo, FOB, Bauru Sch Dent, Dept Dent Endodont & Dent Mat, Alameda Dr Octavio Pinheiro Brisolla 9-75, BR-17012101 Sao Paulo - Brazil

^[3] Ctr Higher Educ Rezende & Potrich FAMA, Mineiros Sch Dent, Dept Pediat Dent, Sao Paulo - Brazil

^[4] Univ Sao Paulo, FOB, Bauru Sch Dent, Dept Operat Dent Endodont & Dent Mat, Alameda Dr Octavio Pinheiro Brisolla 9-75, BR-17012101 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: MICROSCOPY RESEARCH AND TECHNIQUE; v. 79, n. 2, p. 72-80, FEB 2016.
• Citações Web of Science: 5

Resumo

The application of resin-based materials is an alternative of treatment for eroded lesions. Nevertheless, there are no studies about the penetration of these materials into eroded lesion, which might affect its adhesion. Therefore, this study evaluated the penetration of four resin-based materials, with and without enamel etching. By using an in vitro protocol, types of treatment were studied at five levels (AdheSE (R), Tetric N-Bond (R), Single Bond 2 (R), Helioseal Clear (R), Icon (R)) and types of enamel etching in two levels (with and without). Materials were stained with 0.02 mg/mL ethanolic solution of tetramethylrhodamine isothiocyanate. Bovine enamel samples (4 3 4 mm) were immersed in 0.01 M HCl, pH 2.3, for 30 seconds to produce initial eroded lesions. Afterward, the materials were applied on half of sample enamel surface following the manufacturer's instructions. On the other half of sample, the materials were applied without etching the enamel. Materials penetration into the enamel was assessed by Confocal Laser Scanning Microscopy on reflection and fluorescence modes. The penetration depth (PD) was measured using ImageJ software. Data were analyzed by two-way ANOVA and Tukey test (P<0.05). Regardless of the material, etched enamel resulted in higher PD than non-etched (P<0.05). Icon (R) showed the highest PD in enamel followed by Helioseal Clear (R) (P<0.05), with significant difference between them (P<0.05) and no difference was found among AdheSE (R), Tetric N-Bond (R), and Single Bond 2 (R) (P>0.05). It can be concluded that prior enamel etching increased the materials penetration into eroded enamel and the Icon (R)-infiltrant presented highest penetration. Microsc. Res. Tech. 79: 72-80, 2016. (C) 2015 Wiley Periodicals, Inc. (AU)

• Processo FAPESP: 11/12395-4 - Efeito da aplicação de infiltrante sobre lesões iniciais de erosão e posterior submissão a desafio erosivo: estudo in vitro
• Beneficiário: Ana Paula Boteon
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica